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Molecular Biology

Questions related to PCR, cloning, DNA, RNA ...

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Topic	Stats	Last Post Info
what is the ORFeome clone? Started by lna, 17 Mar 2013 ORF, cloning	2 replies 165 views	lna 18 Mar 2013
No amplification during RT-PCR Started by rmbio, 16 Mar 2013 cDNA synthesis problem?	4 replies 149 views	Rajpoot 17 Mar 2013
rescue cloning of a transposon mutant Started by shawn6022, 15 Mar 2013 transposon mutagenesis and 1 more...	2 replies 124 views	shawn6022 17 Mar 2013
Dephosphorylation Started by lucilius, 15 Mar 2013	7 replies 158 views	lucilius 17 Mar 2013
His tag introduction in to gene and primer design Started by Always_student, 10 Mar 2013 His tag, primer design	2 replies 184 views	Always_student 15 Mar 2013
study of receptors in cell lines Started by SF_HK, 15 Mar 2013	1 reply 139 views	GNANA 15 Mar 2013
Production of GST tagged fusion proteins in BL21 Star cells Started by RVCNick, 19 Feb 2013 Help	2 replies 208 views	Pangea 13 Mar 2013
Mouse genotyping issues Started by LacquerHead, 13 Mar 2013	1 reply 126 views	bob1 13 Mar 2013
In-situ hybridization Started by Mad researcher, 12 Mar 2013 in-situ hybridization and 1 more...	1 reply 138 views	DrRupesh Kumar Rajput 13 Mar 2013
RNA gel electrophoresis for integrity check - denaturing or non-denaturing?? Started by Neanderthal, 07 Mar 2013 RNA gel electrophoresis and 4 more...	6 replies 184 views	Neanderthal 12 Mar 2013
qPCR multiplex- impact of the fluorophore on probe annealing temperature Started by dan_, 08 Mar 2013	1 reply 112 views	jerryshelly1 10 Mar 2013
Help: Best way to disrupt blood cells to generate blood debris and gDNA to be us Started by Fluoresce, 09 Mar 2013 Blood, debris, Detergent free and 1 more...	4 replies 161 views	Fluoresce 10 Mar 2013
Principle of silica membrane binding of nucleic acid Started by xxcici, 09 Mar 2013	6 replies 167 views	phage434 10 Mar 2013
Placental RNA extraction with qiagen rneasy mini plus kit Started by sheon, 05 Mar 2013	9 replies 186 views	bob1 10 Mar 2013
genogroup and genotype Started by xxcici, 09 Mar 2013	2 replies 102 views	xxcici 09 Mar 2013
How double stranded DNA becomes single stranded in LAMP assay? Started by xxcici, 08 Mar 2013	3 replies 104 views	xxcici 09 Mar 2013
Expression from T7 promoter/DH5 Alpha cells Started by Kumar@Reading, 08 Mar 2013 T7 promoter	0 replies 128 views	Kumar@Reading 08 Mar 2013
expression of promoter T7 Started by Hoanguyen_TQ, 19 Jun 2011	6 replies 613 views	Kumar@Reading 08 Mar 2013
Cloning at 6 kb gene Started by praveen921, 07 Mar 2013	3 replies 129 views	mdfenko 08 Mar 2013
the mechanism for microorganism replicating their genome without primer in vivo Started by xxcici, 08 Mar 2013	1 reply 96 views	hobglobin 08 Mar 2013
Beta alanine alternative? Started by Praitibha Batra, 08 Mar 2013 molecular biology	2 replies 85 views	mdfenko 08 Mar 2013
lncRNA full-sequence or partial sequence Started by julieta, 07 Mar 2013 non-coding RNA, cloning, ncRNA and 1 more...	2 replies 108 views	julieta 07 Mar 2013
Trouble with DNA purity (A260/A280) Started by Alli, 05 Mar 2013 DNA purity Maxi	3 replies 134 views	mdfenko 05 Mar 2013
Advice needed to subclone big inserts (6 kb) into pET system Started by kpchai, 27 Feb 2013 Subcloning, big insert and 1 more...	2 replies 116 views	neuron 05 Mar 2013
Luciferase assay - extreme variation in RLU Started by Pkrish, 01 Mar 2013	1 reply 119 views	bob1 02 Mar 2013