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Molecular Biology

Questions related to PCR, cloning, DNA, RNA ...

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Topic	Stats	Last Post Info
problems with protein expression (or transfection) from Gateway plasmids? Started by lna, 15 Apr 2013	0 replies 180 views	lna 15 Apr 2013
DNA extraction from parafin sections Started by lula, 15 Apr 2013	0 replies 182 views	lula 15 Apr 2013
Plasmid isolation from gram positive bacteria Started by 2xzwei, 10 Apr 2013	2 replies 181 views	2xzwei 15 Apr 2013
Deleting restriction enzyme sites Started by LacquerHead, 12 Apr 2013	1 reply 290 views	bob1 12 Apr 2013
Determining in frame in modified vector Started by LacquerHead, 12 Apr 2013	1 reply 238 views	bob1 12 Apr 2013
Are two promoters needed for co-expressing 2 proteins from the same plasmid in S Started by donny, 08 Apr 2013	4 replies 346 views	donny 12 Apr 2013
Creating primers to add restriction sites to vector backbone Started by LacquerHead, 11 Apr 2013	7 replies 204 views	phage434 12 Apr 2013
cDNA test on 1% agarose gel Started by xxcici, 11 Apr 2013	3 replies 119 views	bob1 11 Apr 2013
sacB Counter-Selection to generate double cross-over deletion mutants Started by gandhi, 20 Mar 2013 sacB, Allelic Exchange and 3 more...	3 replies 200 views	gandhi 10 Apr 2013
How to check for the presence of plasmid? Started by mimiW, 05 Apr 2013	3 replies 280 views	2xzwei 10 Apr 2013
TA Cloning of alpha-1,3 glucanase: No blue colonies Started by sfora71, 19 Mar 2013	5 replies 281 views	bob1 09 Apr 2013
rsaI enzyme Started by Lay La, 05 Apr 2013	1 reply 306 views	bob1 05 Apr 2013
Extracting total RNA from woody plant roots Started by cinnamon wattle, 01 Apr 2013 RNA, shrub roots	2 replies 468 views	cinnamon wattle 04 Apr 2013
P1 transduction: What will happen if more than 2 FRT sequences exist Started by Qundo12, 03 Apr 2013 P1 transduction, E. coli and 2 more...	0 replies 221 views	Qundo12 03 Apr 2013
Quantify DNA in gel electrophoresis bands Started by Sylvia Joanne, 26 Mar 2013 gel electrophoresis, quantify	7 replies 1,242 views	Sylvia Joanne 28 Mar 2013
Quantify total DNA in small cell lysate...? Started by assembler01, 27 Mar 2013 quantify, DNA, lysate	1 reply 713 views	bigudukaz 28 Mar 2013
camelpox DNA extraction Started by nightingale, 27 Mar 2013 help	0 replies 164 views	nightingale 27 Mar 2013
getting strange data in Luciferase-renilla assay Started by gingerx, 21 Mar 2013 luciferase-renilla	2 replies 166 views	gingerx 25 Mar 2013
Protein extraction and isolectric focusing Started by SailorNorway, 24 Mar 2013	1 reply 156 views	SailorNorway 24 Mar 2013
problem with gene knockout by homologous recombination: crossover always happen Started by Lynn Shay, 22 Mar 2013	0 replies 145 views	Lynn Shay 22 Mar 2013
Homozygote DNA but Heterozygote RNA? Started by Norway, 21 Mar 2013	2 replies 122 views	Norway 21 Mar 2013
PCR product running on agarose gel Started by vanu, 20 Feb 2013 1 2 3	Hot 32 replies 1,154 views	vanu 21 Mar 2013
ligation with one ristrection site/enzyme Started by yusii, 16 Mar 2013 ristriction digest, ligation and 2 more...	3 replies 228 views	2xzwei 20 Mar 2013
site-directed mutagenesis Started by zzha158, 28 Feb 2013 1 2	Hot 20 replies 375 views	GNANA 20 Mar 2013
What is the necessary time for lipofectamin to make complex with pre-miRNA oligo Started by gingerx, 19 Mar 2013 transfection, miRNA	1 reply 170 views	bob1 20 Mar 2013