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Molecular Biology

Questions related to PCR, cloning, DNA, RNA ...

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Topic	Stats	Last Post Info
SOC vs TB in transformations Started by LacquerHead, 26 Feb 2013	1 reply 128 views	Trof 27 Feb 2013
TCA precipitation Started by biology_06er, 26 Feb 2013	1 reply 124 views	biology_06er 27 Feb 2013
Best way to isolate viral mRNA for RT-PCR? Started by ACN, 25 Feb 2013 Isolating mRNA, mRNA, RT-PCR and 2 more...	4 replies 197 views	ACN 26 Feb 2013
Length Limitation of Universal Base Homopolymer Started by MrFreak, 25 Feb 2013 DNA, homopolymer, Universal Base and 2 more...	0 replies 142 views	MrFreak 25 Feb 2013
Continuous growth of E. coli on AMP LB plate Started by tihong10, 05 Feb 2013 e. coli, lawn, amp	2 replies 196 views	tihong10 25 Feb 2013
Single RE site cloning Started by LacquerHead, 24 Feb 2013	3 replies 137 views	GNANA 25 Feb 2013
What is the longest mRNA imported into mitochondria? Started by cellthetruth, 25 Feb 2013 mRNA, mitochondria, import and 1 more...	0 replies 125 views	cellthetruth 25 Feb 2013
contamination of human cell lines with yeast Started by perunica, 11 Dec 2012 saccharomyces, yeast	3 replies 343 views	Multiplexion 22 Feb 2013
Problem with Tamoxifen inducible Cre mouse Started by sprout, 21 Feb 2013 knockout mouse, transgenic mouse and 2 more...	0 replies 162 views	sprout 21 Feb 2013
Western difficulty in primary neuron cells. Started by Fatsheep, 04 Dec 2010	1 reply 632 views	GNANA 20 Feb 2013
BL21 competent cells and T7 RNA polymerase Started by LacquerHead, 19 Feb 2013	0 replies 153 views	LacquerHead 19 Feb 2013
Blue fluorescent color of agarose gel in UV Started by neuron, 14 Feb 2013	7 replies 193 views	neuron 19 Feb 2013
Transformation problem Started by Christi, 17 Feb 2013 transformation, dh5a, top10	5 replies 232 views	phage434 18 Feb 2013
PCR amplification with high fidelity enzyme Started by Suzanna, 18 Feb 2013	1 reply 131 views	hobglobin 18 Feb 2013
Can anyone vouch for a protocol to get good gDNA from fungi? Started by bjk1985, 12 Feb 2013	2 replies 172 views	bjk1985 16 Feb 2013
Successful Transformation->Getting Colongy--> Getting DNA after miniprep Started by neoplazi, 11 Feb 2013 Transformastion	9 replies 285 views	neoplazi 15 Feb 2013
finding gene sequence to delete Started by lmg, 14 Feb 2013	4 replies 161 views	lmg 15 Feb 2013
Staining lysed cells in Drosophila Started by levuccio, 14 Feb 2013 lysed, drosophila, phage, virus	0 replies 161 views	levuccio 14 Feb 2013
heterologous gene integration into e.coli genome Started by yja97, 13 Feb 2013	1 reply 135 views	neuron 13 Feb 2013
delaying RNA extraction; using buffer RLT to disrupt cells and place on ice? Started by rumjon, 13 Feb 2013 RNA, RLT, PBMCs	3 replies 208 views	rumjon 13 Feb 2013
survival strategy of bacteria Started by Anula, 11 Jan 2013 survive, bacteria, macrophages	4 replies 271 views	Aiken5 12 Feb 2013
Qiagen column eluted DNA gives higher concetration than the original sample Started by Fluoresce, 09 Feb 2013	10 replies 283 views	Fluoresce 12 Feb 2013
improper recombination in transgenic mice Started by sligeach, 11 Dec 2012	1 reply 295 views	garzaj9221 08 Feb 2013
Why do only some nuclear lysis buffers create DNA 'goop' Started by cbc625, 07 Feb 2013 DNA, nuclear lysis buffer	1 reply 172 views	Curtis 07 Feb 2013
cDNA or genomic DNA for over expression in Arabidopsis Started by fighter, 07 Feb 2012	1 reply 514 views	Taylor125 07 Feb 2013