Molecular Biology

PCR product smearing on gel

Started by kudna, 03 Sep 2004   pcr, smearing, gel

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Reuse DNA spin column

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Blunt-cloning -all are vector self-ligation products?

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Genome walking

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How to ligate ds-oligos into a vector?

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Genomic DNA contamination of RNA

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cloning from PCR

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Double digestion and ligation problem

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Handling RNA - How to avoid RNase contamination

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Barcoding PCR products

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Digested PCR product migrate slower than uncut

Incorporate biotinylated-dNTPs by a polymerase

Dephosphorylation of vector before ligation

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Need Help: Bacterial gene deletion- sacB sucrose resistance

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Why is DTT used in reverse transcription (RT) reaction?

T4 RNA ligase

The significance of the 260/230 ratio for nucleic acid purity

RNA extraction using TRIzol

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Molecular Biology Book

ISH sense control appears to completely stain the nucleus

Started by alsospade, 25 Jul 2014   ISH, in situ hybridization, probe and 2 more...

How to detect protein-RNA Interaction

Colony PCR

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about DNA isolation from blood clots

Transformation problem. Please help!

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