Molecular Biology

PCR product smearing on gel

Started by kudna, 03 Sep 2004   pcr, smearing, gel

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Blunt-cloning -all are vector self-ligation products?

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Reuse DNA spin column

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Genome walking

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How to ligate ds-oligos into a vector?

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Genomic DNA contamination of RNA

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cloning from PCR

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Double digestion and ligation problem

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Handling RNA - How to avoid RNase contamination

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Digested PCR product migrate slower than uncut

Barcoding PCR products

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Incorporate biotinylated-dNTPs by a polymerase

Dephosphorylation of vector before ligation

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Need Help: Bacterial gene deletion- sacB sucrose resistance

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The significance of the 260/230 ratio for nucleic acid purity

T4 RNA ligase

Molecular Biology Book

Why is DTT used in reverse transcription (RT) reaction?

How to detect protein-RNA Interaction

RNA extraction using TRIzol

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GST Pull down Assay

Colony PCR

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about DNA isolation from blood clots

Big insert to put back into the vector

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Tris-acetate buffer?