Molecular Biology

Blunt-cloning -all are vector self-ligation products?

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PCR product smearing on gel

Started by kudna, 03 Sep 2004   pcr, smearing, gel

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Genome walking

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Genomic DNA contamination of RNA

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How to ligate ds-oligos into a vector?

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Reuse DNA spin column

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cloning from PCR

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Double digestion and ligation problem

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Barcoding PCR products

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Handling RNA - How to avoid RNase contamination

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Cloning before sequencing- is it necessary???

Digested PCR product migrate slower than uncut

Changing the vector

Started by RNA woman, 05 Aug 2014   cloning

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Nde1-BamH1 makes me sad

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Multiplex PCR

Started by Mad researcher, 17 Oct 2012   multiplex PCR, amplification and 1 more...

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PCR product running on agarose gel

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Need Help: Bacterial gene deletion- sacB sucrose resistance

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Help with Lysis

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Ligation problem: clones survive selection but nothing in there?

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amplified proviral genome and got human sequence

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CTAB of Fungi

Started by Axolotl9250, 11 Jul 2012   CTAB, DNA, Extraction and 2 more...

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DNA extraction impurities

Started by sayk, 02 Nov 2013   dna extraction, promega and 1 more...

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small sized restricted bands couldnt be visualized

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Dephosphorylation of vector before ligation

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ligation and transformation problems! Urgent!

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