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Molecular Biology
Questions related to PCR, cloning, DNA, RNA ...
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Barcoding PCR productsStarted by GradMicro, 11 Dec 2012 |
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PCR product smearing on gelStarted by kudna, 03 Sep 2004  pcr, smearing, gel
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Genomic DNA contamination of RNAStarted by bp_serene, 10 Jun 2009 |
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Blunt-cloning -all are vector self-ligation products?Started by karen, 11 Jul 2003 |
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Double digestion and ligation problemStarted by ixsix, 17 May 2004 |
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Genome walkingStarted by kiwi, 02 Sep 2004 |
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How to ligate ds-oligos into a vector?Started by kroger, 25 Aug 2004 |
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cloning from PCRStarted by dorightman, 24 Jun 2004 |
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Digested PCR product migrate slower than uncutStarted by lucabag, 07 Mar 2011 |
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Handling RNA - How to avoid RNase contaminationStarted by susanna, 24 Nov 2009 |
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Reuse DNA spin columnStarted by Minnie Mouse, 03 Feb 2009 |
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Cloning before sequencing- is it necessary???Started by Ameya P, 13 Sep 2010 |
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Odd issue converting mutiplex PCR to single reactionsStarted by MealyWorm, Yesterday, 07:54 AM PCR, multiplex PCR, Multiplexing and 2 more...
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What is your favorite method for getting inserts into vectors?Started by labtastic, 22 Jul 2017 |
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Possibly a stupid question.Started by Doraid, 20 Jul 2017 |
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EMSA troubleshootingStarted by DrSnood, 27 Jun 2017 EMSA, DNA binding, NF-kB
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Preservation of RNA/cDNA samplesStarted by Nephrite, 03 Jun 2017 |
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Question about CaCl2 and CaCl2Started by Doraid, 05 Jun 2017 |
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Dilution helpStarted by Janarthanan P, 06 Jun 2017 gene fragment dilution and 1 more...
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Cycloheximide concentrationStarted by Doraid, 25 May 2017 |
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Cell cycleStarted by SF_HK, 08 May 2017 |
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wnt/beta catenin signallingStarted by SF_HK, 18 Jan 2017 |
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Very low yields for >10kb fragment gel extractionsStarted by naglemi, 18 Feb 2017 dna, purification, gel extraction and 1 more...
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Design of <=40bp primers adding restriction sites and also Gibson Assembly oStarted by naglemi, 18 Feb 2017 cloning, gibson assembly
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Using RNA minikit for DNA extractionStarted by GradMicro, 09 Jan 2017 |
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