Molecular Biology

Barcoding PCR products

• 1
• 2

PCR product smearing on gel

Started by kudna, 03 Sep 2004   pcr, smearing, gel

• 1
• 2

Genomic DNA contamination of RNA

• 1
• 2

Blunt-cloning -all are vector self-ligation products?

• 1
• 2

Double digestion and ligation problem

• 1
• 2

Genome walking

• 1
• 2

How to ligate ds-oligos into a vector?

• 1
• 2

cloning from PCR

• 1
• 2

Digested PCR product migrate slower than uncut

Handling RNA - How to avoid RNase contamination

• 1
• 2

Reuse DNA spin column

• 1
• 2

Cloning before sequencing- is it necessary???

a half less of plasmid copy number in the solution resulting from ddPCR than qPC

Started by cartclear, 16 Aug 2016   ddpcr, qpcr, copy number

Need to run gel after miniprep before digest?

Qiagen ATL, AL, AW1, AW2 recipes?

Started by Meta, 07 Jul 2016   dna extraction qiagen

Good diluents for RNA

solution preparation

concentration of starting material

site directed mutagenesis

site directed mutagenesis

DNA concentration

How to improve the purity of total RNA isolated from mouse conjunctiva