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Questions related to PCR, cloning, DNA, RNA ...
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Barcoding PCR productsStarted by GradMicro, 11 Dec 2012 |
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PCR product smearing on gelStarted by kudna, 03 Sep 2004  pcr, smearing, gel
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Genomic DNA contamination of RNAStarted by bp_serene, 10 Jun 2009 |
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Blunt-cloning -all are vector self-ligation products?Started by karen, 11 Jul 2003 |
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Double digestion and ligation problemStarted by ixsix, 17 May 2004 |
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Genome walkingStarted by kiwi, 02 Sep 2004 |
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How to ligate ds-oligos into a vector?Started by kroger, 25 Aug 2004 |
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cloning from PCRStarted by dorightman, 24 Jun 2004 |
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Digested PCR product migrate slower than uncutStarted by lucabag, 07 Mar 2011 |
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Handling RNA - How to avoid RNase contaminationStarted by susanna, 24 Nov 2009 |
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Reuse DNA spin columnStarted by Minnie Mouse, 03 Feb 2009 |
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Cloning before sequencing- is it necessary???Started by Ameya P, 13 Sep 2010 |
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ScyTek - ESTABLISHING HUMAN PROSTATE CANCER CELL XENOGRAFTS IN BONEStarted by scyteklab, 09 Aug 2018 Cancer, Cell, Bone, PROSTATE and 1 more...
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Duplication of ITS-5.8s Amplicon (following Sanger sequencing and nBLAST)Started by R1012, 24 Jul 2018 |
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Gene promoter - Luciferase reporter assay helpStarted by Natalia KM, 10 Jul 2018 gene promoter reporter
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Difficult DNA extraction-collagen mutant nematode refusing to give up its DNAStarted by worm1975, 12 Jul 2018 DNA extraction, nematode and 1 more...
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String of A's (15-20nt) w/out polyA signal in middle of rlentiviral vector;Started by Beaver Aggie, 26 Jun 2018 Lentivirus and 1 more...
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CholesterolStarted by rfm1234, 29 Jun 2018 |
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Weak band in negative control in PCRStarted by liligompac, 27 May 2018 |
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multiple melting curvesStarted by keegi, 07 Oct 2016 |
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Cloning of big insertsStarted by EvelyneR, 23 Jan 2017 cloning
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Very low yields for >10kb fragment gel extractionsStarted by naglemi, 18 Feb 2017 dna, purification, gel extraction and 1 more...
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Viscous plasmids and transfection issuesStarted by Trof, 09 Jan 2018 |
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Crude lysis Plasmid isolationStarted by D.Luffy, 04 May 2018 plasmid isolation
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Favorite polymerase for general cloning?Started by DrSnood, 17 Apr 2018 |
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