Today I will inspect the new HeLa cells that I awoke yesterday, proceed with infecting 3T3 cells (RE: a plaque assay) to gain the titer of four different aliquots of the mCMV strain, & turn in my progress report. Read Full Entry
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Today I will inspect the new HeLa cells that I awoke yesterday, proceed with infecting 3T3 cells (RE: a plaque assay) to gain the titer of four different aliquots of the mCMV strain, & turn in my progress report. Read Full Entry
My stipend was stopped 3 months ago, because the last research grant expired.
Now I'm on my own, not funded, and I have to fork-out money to pay my semester fees.
The next semester starts coming August, but I do not have money even to pay my next month room rental.
I should have finished my project by now, however due to unavoidable... Read Full Entry
Now I'm on my own, not funded, and I have to fork-out money to pay my semester fees.
The next semester starts coming August, but I do not have money even to pay my next month room rental.
I should have finished my project by now, however due to unavoidable... Read Full Entry
The recent passing of one of my scientific heroes, Benoît Mandelbrot, the overly large sports section, coupled with the distinct lack of science news in the newspaper this morning has me thinking... Where are our science heroes these days?
It seems to me that in the mid... Read Full Entry
It seems to me that in the mid... Read Full Entry
Dear Blog,
It has been a case of "I did warn you."
I have attempted to improve the efficiency of my multiway ligation by using a different ligase, a higher fidelity ligase. The only other ligase sold commercially is E.coli ligase. It is stated that E. coli does have higher fidelity compared to T4 ligase, however NEB did... Read Full Entry
It has been a case of "I did warn you."
I have attempted to improve the efficiency of my multiway ligation by using a different ligase, a higher fidelity ligase. The only other ligase sold commercially is E.coli ligase. It is stated that E. coli does have higher fidelity compared to T4 ligase, however NEB did... Read Full Entry
Size determination of unknown protein using the Ferguson plot
During electrophoresis in SDS-polyacrylamide gels there is a linear relationship between the logarithm of the molecular weight and the relative distance of the migration of the SDS-polypeptide micelle.
To calculate the molecular weight of an unknown sample or to identify the... Read Full Entry
During electrophoresis in SDS-polyacrylamide gels there is a linear relationship between the logarithm of the molecular weight and the relative distance of the migration of the SDS-polypeptide micelle.
To calculate the molecular weight of an unknown sample or to identify the... Read Full Entry
as said before, I'd try to do a bit more blogging, and so this is my second post here... the title refers to the situation in my home country, Mexico, which, if you read regularly the newspaper, you might have read on the headlines lately.
It's been almost two years I have been away from it, and it feels like it has changed enormously... Read Full Entry
It's been almost two years I have been away from it, and it feels like it has changed enormously... Read Full Entry
How to be an unsuccessful single:
* See being single as a condition that needs to be cured.
* Be on a constant lookout for that one person that is going to make everything right.
* Put off living your life until you meet someone.
* Believe that no matter what you do, you will wind up hurt.
* Resign yourself to being alone and... Read Full Entry
* See being single as a condition that needs to be cured.
* Be on a constant lookout for that one person that is going to make everything right.
* Put off living your life until you meet someone.
* Believe that no matter what you do, you will wind up hurt.
* Resign yourself to being alone and... Read Full Entry
Many people have problem identifying or predicting the promoter sequence of a gene, or don't know how to get the actual sequence for analysis such as primer design, transcription factor binding site search, etc. Here I provide ways how I do these things.
1. How to find and retrieve promoter sequences from genome databases
Promoter... Read Full Entry
1. How to find and retrieve promoter sequences from genome databases
Promoter... Read Full Entry
ABI 3730xl Setup & DNA Sample Loading
NOTE: See Spring 2005 Instrument Configurations for sample plate loading matrix
3.1 Start the Instrument:
NOTE: In most cases, the instrument will be running. Sample loading will begin at step d below.
a. On the instrument, ensure that the:
i. Oven door is closed.
ii. ... Read Full Entry
NOTE: See Spring 2005 Instrument Configurations for sample plate loading matrix
3.1 Start the Instrument:
NOTE: In most cases, the instrument will be running. Sample loading will begin at step d below.
a. On the instrument, ensure that the:
i. Oven door is closed.
ii. ... Read Full Entry
So it's just before lunch and for the first time ever, I'm entering in a blog. Honestly, it's difficult: there is no way to hear intonation or sarcasm. My hat is tipped to other famous bloggers as they are able to do so adeptly, I presume.
Anyway, I'm in the midst of trying to plan a traditional Indian wedding in the US, while... Read Full Entry
Anyway, I'm in the midst of trying to plan a traditional Indian wedding in the US, while... Read Full Entry
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