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trying to get a nice curve to pick a subtoxic dose of atorvastatin; I did the cytotoxicity assay on 3D AC16 cardiac cells

Posted by sun shine 411 in sun shine 411's Blog, 18 September 2021 - - - - - - · 1,459 views

I am trying to get a nice curve to pick a subtoxic dose of atorvastatin; I did the cytotoxicity assay on 3D AC16 cardiac cells from concentration 30 uM to 0.001 uM. However, I did it so many times, and it is the same, and at 3uM gave 100 % cell viability, and then it went down. Why is that happing?. Logically because it is a serial dilution and the concen...

Potential Way for Hair Regeneration Was Found

Posted by Samantha965 in Samantha965's Blog, 21 April 2015 - * * * * * · 12,585 views

When speaking of baldness, most of the people might think of embarrassment. For some people, baldness is really an annoying problem. Scientists are working to figure out an efficient treatment to this all along the time. Kinds of methods are coming into light. Recently, a novel way was found by scientists after conducted a number of researches. This metho...

LaTeX and a biological thesis -- part 2

Posted by bob1 in bob1's Blog, 16 May 2014 - - - - - - · 9,883 views
Latex, reference managers and 1 more...

In this post I would like to talk about not just LaTeX but some of the software front-ends associated with it, specifically LaTeX editors and reference managers (note: these are all freeware).
For those not familiar with the terminology, front-ends are those bits of software that allow you to access a deeper level of software to do a purpose. ...

Phase 1: Cell membrane

Posted by caters in My Cell from scratch, 15 February 2014 - - - - - - · 11,595 views

Membrane comes before nucleus as you can see here. I have made a spherical liposome and am now looking up membrane proteins, their DNA sequences including the chromosome they are on, and their gene expression.http://upload.wikimedia.org/wikipedia/commons/thumb/d/da/Cell_membrane_detailed_diagram_en.svg/877px-Cell_membrane_detailed_diagram_en.svg.png

Life outside academia

Posted by toejam in toejam's Blog, 05 July 2013 - - - - - - · 9,668 views
private industry, postdoc and 1 more...

hey guys!

It feels like it's been so long since last time I stepped foot on this place (with fungus and all :)) The last three months have been a complete change in my life. Now I'm finally working paying taxes and it's completely different to living as a student. As everything in life, it has its pros and cons.

It was my birthday that...

Early Morning

Posted by ACN in ACN's Blog, 02 June 2013 - - - - - - · 8,479 views
mRNA, virus, sendai, Rt-PCR and 1 more...

I'm preforming an qRT-PCR experiment, to investigate the very first proteins expressed during the early stages of a Sendai virus infection. This morning I am collecting my Mock-infected plates.

I just thought I'd share some...

New entry

Posted by baaran in Try to Get along with this Situation, 30 September 2012 - - - - - - · 126,142 views

Hi all
This is my Fisrt Time Blogging here, You know the Reason I Decided to Write here is that Just an hour ago, a new responsibility was added to mine. Beside Teaching and some other works, I hvae to Work in EDO of the School of Medicine from now on. So I wont be here, in my Office taht is Shared with Tow other persons. I will go to new office that is...

Final year of Phd

Posted by lsek in lsek's Blog, 14 August 2012 - - - - - - · 105,125 views

How time flies, this is my final year of Phd and I can't wait to finish it. I have not visited this site for some time and I do hope that in time ahead I will be more active in this Bioforum and BioBlog.

That's it.

Posted by Adrian K in adrian kohsf's Blog, 14 June 2011 - - - - - - · 101,653 views

My stipend was stopped 3 months ago, because the last research grant expired. Now I'm on my own, not funded, and I have to fork-out money to pay my semester fees.The next semester starts coming August, but I do not have money even to pay my next month room rental.I should have finished my project by now, however due to unavoidable situation (by those...

E. coli ligase is not the way

Posted by perneseblue in Perneseblue's Corner, 01 September 2010 - - - - - - · 99,319 views
multiway ligation

Dear Blog,

It has been a case of "I did warn you."

I have attempted to improve the efficiency of my multiway ligation by using a different ligase, a higher fidelity ligase. The only other ligase sold commercially is E.coli ligase. It is stated that E. coli does have higher fidelity compared to T4 ligase, however NEB did warn that...

Size determination of unknown protein using the Ferguson plot

Posted by isabellaweng in isabellaweng's Blog, 31 August 2010 - - - - - - · 101,869 views

Size determination of unknown protein using the Ferguson plot

During electrophoresis in SDS-polyacrylamide gels there is a linear relationship between the logarithm of the molecular weight and the relative distance of the migration of the SDS-polypeptide micelle.
To calculate the molecular weight of an unknown sample or to identify the correct...

Unsuccessful Versus Successful Single

Posted by angeliejohnson in angeliejohnson's Blog, 06 August 2010 - * * * * * · 98,870 views

How to be an unsuccessful single:

    * See being single as a condition that needs to be cured.
    * Be on a constant lookout for that one person that is going to make everything right.
    * Put off living your life until you meet someone.
    * Believe that no matter what you do, you will wind up hurt.
    * Resign yourself to being alone and miserable...

From: How to find promoter sequences

Posted by pcrman in Small RNA blog, 06 August 2010 - - - - - - · 96,327 views
promoter, sequence, database

Many people have problem identifying or predicting the promoter sequence of a gene, or don't know how to get the actual sequence for analysis such as primer design, transcription factor binding site search, etc. Here I provide ways how I do these things. 1. How to find and retrieve promoter sequences from genome databases Promoter sequences are usually t...

3730xl Analyzer Protocol

Posted by shinegene in shinegene's Blog, 01 June 2009 - - - - - - · 7,121 views
molcecular biology

ABI 3730xl Setup & DNA Sample Loading

NOTE: See Spring 2005 Instrument Configurations for sample plate loading matrix

3.1              Start the Instrument:

NOTE: In most cases, the instrument will be running.  Sample loading will begin at step d below.

a.       On the instrument, ensure that the:

i.        Oven door is closed.

ii.      Instrument...

here goes nothing

Posted by labrat612 in labrat612's Blog, 19 February 2009 - - - - - - · 6,585 views

So it's just before lunch and for the first time ever, I'm entering in a blog. Honestly, it's difficult: there is no way to hear intonation or sarcasm. My hat is tipped to other famous bloggers as they are able to do so adeptly, I presume.

Anyway, I'm in the midst of trying to plan a traditional Indian wedding in the US, while maintaining...

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