Create a custom theme
0
literature list of gene synthesis
Posted by shinegene,
01 June 2010
·
639 views
molcecular biology
Gao X, Yo P, Keith A, Ragan TJ, Harris TK: Thermodynamically balanced inside-out (TBIO) PCR-based gene synthesis: a novel method of primer design for high-fidelity assembly of longer gene sequences.
Nucleic acids research 2003, 31(22):e143. PubMed Abstract | Publisher Full Text | PubMed Central Full Text
Stemmer WP, Crameri A, Ha KD, Brennan TM, Heyneker HL: Single-step assembly of a gene and entire plasmid from large numbers of oligodeoxyribonucleotides.
Gene 1995, 164(1):49-53. PubMed Abstract | Publisher Full Text
Xiong AS, Yao QH, Peng RH, Duan H, Li X, Fan HQ, Cheng ZM, Li Y: PCR-based accurate synthesis of long DNA sequences.
Nat Protoc 2006, 1(2):791-797. PubMed Abstract | Publisher Full Text
Xiong AS, Yao QH, Peng RH, Li X, Fan HQ, Cheng ZM, Li Y: A simple, rapid, high-fidelity and cost-effective PCR-based two-step DNA synthesis method for long gene sequences.
Nucleic acids research 2004, 32(12):e98. PubMed Abstract | Publisher Full Text | PubMed Central Full Text
Jones DH, Howard BH: A rapid method for recombination and site-specific mutagenesis by placing homologous ends on DNA using polymerase chain reaction.
Biotechniques 1991, 10(1):62-66. PubMed Abstract
Wu G, Wolf JB, Ibrahim AF, Vadasz S, Gunasinghe M, Freeland SJ: Simplified gene synthesis: A one-step approach to PCR-based gene construction.
Journal of Biotechnology 2006, 124(3):496-503. PubMed Abstract | Publisher Full Text
Young L, Dong Q: Two-step total gene synthesis method.
Nucleic acids research 2004, 32(7):e59. PubMed Abstract | Publisher Full Text | PubMed Central Full Text
Current Protocols in Molecular Biology UNIT 8.2B Gene Synthesis: Assembly of Target Sequences Using Mutually Priming Long Oligonucleotides
David D. Moore1
1Massachusetts General Hospital, Boston, Massachusetts
Copyright © 2003 John Wiley & Sons, Inc. All rights reserved.
DOI: 10.1002/0471142727.mb0802bs26
Online Posting Date: May, 2001
Print Publication Date: April, 1994
Abstract | Full Text: HTML PDF (123K)
Abstract
This protocol uses pairs of oligonucleotides annealed at a short duplex segment at their 3' ends as both templates and primers (mutually primed synthesis) to generate desired sequences up to 400 bp in a single step. The procedure is divided into three sections: design of the oligonucleotides, extension by mutually primed synthesis, and cloning of the extension products. The strategy for design of oligonucleotides is discussed in the commentary.
Nucleic acids research 2003, 31(22):e143. PubMed Abstract | Publisher Full Text | PubMed Central Full Text
Stemmer WP, Crameri A, Ha KD, Brennan TM, Heyneker HL: Single-step assembly of a gene and entire plasmid from large numbers of oligodeoxyribonucleotides.
Gene 1995, 164(1):49-53. PubMed Abstract | Publisher Full Text
Xiong AS, Yao QH, Peng RH, Duan H, Li X, Fan HQ, Cheng ZM, Li Y: PCR-based accurate synthesis of long DNA sequences.
Nat Protoc 2006, 1(2):791-797. PubMed Abstract | Publisher Full Text
Xiong AS, Yao QH, Peng RH, Li X, Fan HQ, Cheng ZM, Li Y: A simple, rapid, high-fidelity and cost-effective PCR-based two-step DNA synthesis method for long gene sequences.
Nucleic acids research 2004, 32(12):e98. PubMed Abstract | Publisher Full Text | PubMed Central Full Text
Jones DH, Howard BH: A rapid method for recombination and site-specific mutagenesis by placing homologous ends on DNA using polymerase chain reaction.
Biotechniques 1991, 10(1):62-66. PubMed Abstract
Wu G, Wolf JB, Ibrahim AF, Vadasz S, Gunasinghe M, Freeland SJ: Simplified gene synthesis: A one-step approach to PCR-based gene construction.
Journal of Biotechnology 2006, 124(3):496-503. PubMed Abstract | Publisher Full Text
Young L, Dong Q: Two-step total gene synthesis method.
Nucleic acids research 2004, 32(7):e59. PubMed Abstract | Publisher Full Text | PubMed Central Full Text
Current Protocols in Molecular Biology UNIT 8.2B Gene Synthesis: Assembly of Target Sequences Using Mutually Priming Long Oligonucleotides
David D. Moore1
1Massachusetts General Hospital, Boston, Massachusetts
Copyright © 2003 John Wiley & Sons, Inc. All rights reserved.
DOI: 10.1002/0471142727.mb0802bs26
Online Posting Date: May, 2001
Print Publication Date: April, 1994
Abstract | Full Text: HTML PDF (123K)
Abstract
This protocol uses pairs of oligonucleotides annealed at a short duplex segment at their 3' ends as both templates and primers (mutually primed synthesis) to generate desired sequences up to 400 bp in a single step. The procedure is divided into three sections: design of the oligonucleotides, extension by mutually primed synthesis, and cloning of the extension products. The strategy for design of oligonucleotides is discussed in the commentary.
