Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!




- - - - -

literature list of gene synthesis

Posted by shinegene, 01 June 2010 · 1,002 views

molcecular biology
Gao X, Yo P, Keith A, Ragan TJ, Harris TK: Thermodynamically balanced inside-out (TBIO) PCR-based gene synthesis: a novel method of primer design for high-fidelity assembly of longer gene sequences.

Nucleic acids research 2003, 31(22):e143. PubMed Abstract | Publisher Full Text | PubMed Central Full Text


Stemmer WP, Crameri A, Ha KD, Brennan TM, Heyneker HL: Single-step assembly of a gene and entire plasmid from large numbers of oligodeoxyribonucleotides.

Gene 1995, 164(1):49-53. PubMed Abstract | Publisher Full Text





Xiong AS, Yao QH, Peng RH, Duan H, Li X, Fan HQ, Cheng ZM, Li Y: PCR-based accurate synthesis of long DNA sequences.

Nat Protoc 2006, 1(2):791-797. PubMed Abstract | Publisher Full Text




Xiong AS, Yao QH, Peng RH, Li X, Fan HQ, Cheng ZM, Li Y: A simple, rapid, high-fidelity and cost-effective PCR-based two-step DNA synthesis method for long gene sequences.

Nucleic acids research 2004, 32(12):e98. PubMed Abstract | Publisher Full Text | PubMed Central Full Text



Jones DH, Howard BH: A rapid method for recombination and site-specific mutagenesis by placing homologous ends on DNA using polymerase chain reaction.

Biotechniques 1991, 10(1):62-66. PubMed Abstract




Wu G, Wolf JB, Ibrahim AF, Vadasz S, Gunasinghe M, Freeland SJ: Simplified gene synthesis: A one-step approach to PCR-based gene construction.

Journal of Biotechnology 2006, 124(3):496-503. PubMed Abstract | Publisher Full Text




Young L, Dong Q: Two-step total gene synthesis method.

Nucleic acids research 2004, 32(7):e59. PubMed Abstract | Publisher Full Text | PubMed Central Full Text




Current Protocols in Molecular Biology UNIT 8.2B Gene Synthesis: Assembly of Target Sequences Using Mutually Priming Long Oligonucleotides
David D. Moore1
1Massachusetts General Hospital, Boston, Massachusetts
Copyright 2003 John Wiley & Sons, Inc. All rights reserved.
DOI: 10.1002/0471142727.mb0802bs26
Online Posting Date: May, 2001
Print Publication Date: April, 1994
Abstract | Full Text: HTML PDF (123K)
Abstract
This protocol uses pairs of oligonucleotides annealed at a short duplex segment at their 3' ends as both templates and primers (mutually primed synthesis) to generate desired sequences up to 400 bp in a single step. The procedure is divided into three sections: design of the oligonucleotides, extension by mutually primed synthesis, and cloning of the extension products. The strategy for design of oligonucleotides is discussed in the commentary.




September 2014

M T W T F S S
1234567
891011121314
151617181920 21
22232425262728
2930     
Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.