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shinegene's Blog



literature list of gene synthesis

Posted by shinegene, 01 June 2010 - - - - - - · 1,048 views
molcecular biology
Gao X, Yo P, Keith A, Ragan TJ, Harris TK: Thermodynamically balanced inside-out (TBIO) PCR-based gene synthesis: a novel method of primer design for high-fidelity assembly of longer gene sequences.

Nucleic acids research 2003, 31(22):e143. PubMed Abstract | Publisher Full Text | PubMed Central Full Text  


Stemmer WP, Crameri A, Ha KD, Brennan TM,...


template of SCI Submission letter

Posted by shinegene, 01 June 2010 - - - - - - · 4,741 views
molcecular biology
Submission letter
1. Dear Dr. Defendi ML:
I am sending a manuscript entitled by which I should like to submit for possible publication in the journal of - .
Yours sincerely

2. Dear Dr. A:
Enclosed is a manuscript entitled by sb, which we are submitting for publication in the journal of - . We have chosen this journal because it deals with - . We...


3730xl Analyzer Protocol

Posted by shinegene, 01 June 2009 - - - - - - · 1,563 views
molcecular biology
ABI 3730xl Setup & DNA Sample Loading

NOTE: See Spring 2005 Instrument Configurations for sample plate loading matrix

3.1              Start the Instrument:

NOTE: In most cases, the instrument will be running.  Sample loading will begin at step d below.

a.       On the instrument, ensure that the:

i.        Oven door is closed.

ii.      Instrument...


DNA cloning

Posted by shinegene, 02 April 2006 - - - - - - · 852 views
molcecular biology
Introduction

This manual is a compilation of many of the everyday methods used in the average molecular biology laboratory, with emphasis on the techniques for large scale DNA sequencing protocols and DNA sequencing automation techniques. The manual has been written in a protocol format, with little theoretical discussion. For theory and additional...





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