Create a custom theme
0
Interferon Protection assay Day 3 {Inoculation}
Posted by ACN,
31 August 2011
·
3,115 views
virus propagation
Image: picture of my virus propagation
Yesterday (8/30/11) addition of 100ul PBL mIFN (interferon) {in a 5 fold serial dilution fashion} done in 1X DMEM + 10%FBS+PSG.
Today {8/31/11} plates (3) were subjected to 100uls of inoculum (EMCV in 1XDMEM+10%FBS+PSG). “dilution based on previously done kill curve”
Plate#1= 1:2K
Plate#2= 1:4k
Plate#3= 1:8k
Plates were spun at 100g for 20minutes rotated then spun again for another 20min. Let incubate at 37C in 5%CO2 for at least 24hr.
Question:
If smallpox can only survive in humans and has seemed to have existed since humans have. What do you think came first smallpox or humans (“chicken or the egg?” Type of question I know.)
