Today I will inspect the new HeLa cells that I awoke yesterday, proceed with infecting 3T3 cells (RE: a plaque assay) to gain the titer of four different aliquots of the mCMV strain, & turn in my progress report.
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Today I will inspect the new HeLa cells that I awoke yesterday, proceed with infecting 3T3 cells (RE: a plaque assay) to gain the titer of four different aliquots of the mCMV strain, & turn in my progress report.
I'd like to call...
Tags:
Virology,
cell culture,
3T3 cells
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Tags:
virus propagation,
3T3 cells,
CPE
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Image: picture of my virus propagation
Yesterday (8/30/11) addition of 100ul PBL mIFN (interferon) {in a 5 fold serial dilution fashion} done in 1X DMEM + 10%FBS+PSG.
Today {8/31/11} plates (3) were subjected to 100uls of inoculum (EMCV in 1XDMEM+10%FBS+PSG)....
Tags:
virus propagation
1 Comments
Why:
The drug company our lab works for wants to test their antibodies conjugated to IFNs for antiviral activity. My task is to understand how much antibody-IFN is needed to "cure" an organism from a viral infection (specifically viruses which causes...
The drug company our lab works for wants to test their antibodies conjugated to IFNs for antiviral activity. My task is to understand how much antibody-IFN is needed to "cure" an organism from a viral infection (specifically viruses which causes...
First off I would like to start my first blog stating: this summer has been the most helpful (academic wise) than any other. I'm conducting undergraduate research in a virology laboratory at UCI. Its my first time exposed to the life of applied science and I am really enjoying it. My time is spent learning assay's, refining my techniques and...
