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ACN's Blog



Early Morning

Posted by ACN, 02 June 2013 - - - - - - · 1,326 views
mRNA, virus, sendai, Rt-PCR and 1 more...
I'm preforming an qRT-PCR experiment, to investigate the very first proteins expressed during the early stages of a Sendai virus infection. This morning I am collecting my Mock-infected plates.

I just thought I'd share some...


Beginning of the quarter

Posted by ACN, 16 October 2012 - - - - - - · 10,258 views

Just a few pictures so far.

Virus Harvest from embryonic chicken eggs:
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HA assay (Sendai...


8/13/12 New Lab

Posted by ACN, 13 August 2012 - - - - - - · 9,721 views

Was recently accepted into a paid Masters Program in at CSULA! This means more new lab pics.


tailoring of a plaque assay

Posted by ACN, in Virology 09 September 2011 - * * * - - · 8,014 views
plaque assay, 3T3 cell, MCMV
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Today I will inspect the new HeLa cells that I awoke yesterday, proceed with infecting 3T3 cells (RE: a plaque assay) to gain the titer of four different aliquots of the mCMV strain, & turn in my progress report.


Plating 3T3 cells for a plaque assay

Posted by ACN, in Virology 08 September 2011 - - - - - - · 7,909 views
Virology, cell culture, 3T3 cells
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I'd like to call these...



Interferon Protection assay Day 3 {Inoculation}

Posted by ACN, 31 August 2011 - - - - - - · 7,633 views
virus propagation
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Image: picture of my virus propagation

Yesterday (8/30/11) addition of 100ul PBL mIFN (interferon) {in a 5 fold serial dilution fashion} done in 1X DMEM + 10%FBS+PSG.
Today {8/31/11} plates (3) were subjected to 100uls of inoculum (EMCV in 1XDMEM+10%FBS+PSG)....


The Interferon Protection Assay

Posted by ACN, 29 August 2011 - - - - - - · 7,324 views
virus and 3 more...
Why:

The drug company our lab works for wants to test their antibodies conjugated to IFNs for antiviral activity. My task is to understand how much antibody-IFN is needed to "cure" an organism from a viral infection (specifically viruses which causes...


Tomorrow = a new day

Posted by ACN, 11 August 2011 - - - - - - · 6,740 views

First off I would like to start my first blog stating: this summer has been the most helpful (academic wise) than any other. I'm conducting undergraduate research in a virology laboratory at UCI. Its my first time exposed to the life of applied science and I am really enjoying it. My time is spent learning assay's, refining my techniques and understanding...





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