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#91190 Why God Didn't Get Tenure

Posted rkay447 on 02 November 2010 - 11:55 AM

Why God Didn't Get Tenure

1. He had only one major publication.
2. It was in Hebrew.
3. It had no references.
4. It wasn't even published in a refereed journal.
5. Some even doubt he wrote it himself.
6. It may be true that he created the world, but what has he done since then?
7. His cooperative efforts have been quite limited.
8. The scientific community has had a hard time replicating his results.
9. He never applied to the Ethics Board for permission to use human subjects.
10. When one experiment went awry he tried to cover it up by drowning the subjects.
11. When subjects didn't behave as predicted, he deleted them from the sample.
12. He rarely came to class, just told students to read the Book.
13. Some say he had his son teach the class.
14. He expelled his first two students for learning.
15. Although there were only ten requirements, most students failed his tests.
16. His office hours were infrequent and usually held on a mountaintop.

#14111 Creative ways to get my hands antibodies for free (archived)

Posted bioforum on 01 February 2009 - 10:39 AM

Creative ways to get my hands antibodies - ...for free (Feb/20/2006 )

Pages: 1 2 3 4 Next

Hey-

Anyone else have money problems in the lab? I always seem to be mooching from the same people and have some decent ideas that can't be tested without the right antibodies, which we don't have enough money to buy (especially for a non-sure thing)

anyone ever heard of industry giving away small amounts of antibodies to an academic lab? Should I network more? What about starting an underground network of grad students who share reagents? Anyone ever sweet talked a sales rep into a free antibody? You think people on the forum would be willing to share reagents if I gave them my boss's FedEx number?

Anyone else have any creative ideas?
I need to graduate and a few extra reagents wouldn't hurt!

Thanks

-Mountainman-

Some companies such as Santa Cruz give away antibodies if you have cited their antibodies in your publications. Once a friend of mine from a foreign country got an antibody from SC because we (in the US) have cited SC antibodies, although my friend is not among the authors.

-pcrman-

At a university where I used to work, there was a lab on-site that would make antibodies for local labs for relatively cheap - have you checked into that at your university?

Also, my boss at my old company would buy antibodies at lower prices as "samples" - but it was an industrial setting not academia, so I don't know if that would help you.

Finally, are you looking for mono- or polyclonal? I know a start-up lab that makes monoclonal antibodies - in order to get customers they *might* be willing to hand some out. But obviously I can't speak for them, I could only send you in their direction.

-soluene-

I get quite a lot of free stuff. I write a nice email, stating what i'm doing, what i need, how our lab is very very poor . Go on to say, that we want to try the stuff out before we buy, because we are so very very poor . I've got hybond+ paper, sybr green kits, midi prep kits, polymerases, laders, transfection kits.... tried to get a new flow cytomoter for free, but the sales guy didn't budge <_< . Haven't tried for antibodies, but i suspect the same would work. for you see, we are so very, very poor.

Vetticus

-vetticus3-

Hehe....

Thanks guys....I'll keep trying to play the poor impoverished grad student act to Santa Cruz and others. I've tried it before with mild success over the phone....but I guess by email it gives you the opportunity to lay it on really thick. Maybe I'll try that next

Mountainman

-Mountainman-

Hey Poor researchers (esp Vetticus),
Its really useful ideas what you have given to him, and this forum is pretty useful for many researchers who suffer to do their stuffs.. hats off to Bioforum.

But in the current lab where am working now (really poor lab, but all are doctors) they bought ''10 X PBS'' which was shocking for me being a biologist, its just waste of money if one can prepare that. I had been working in another lab earlier where we don't even buy mini preps, struggeled with all conventional methods, alkaline lysis.

keeping going

-Biosaint-

Hey, i might be in a very, very poor lab , but once you get something for free once, they keep on sending you things to try!! It's like small christmasses all through the year.

email definately gives you an advantage. You can go on and on about how your work is so interesting, but you can't afford this new whiz-bang reagent, and that you might be able to pursuade your PI into buying some (giving up the money that would have been spent on buying bread crumbs for dinner :huh: *what?*). if you could just try it out, and show your PI that it works, and is worth the expense. After all, it is such a wonderful product, and although no other lab where you work uses it (that's why you can't steal a few uL from them), you've heard it cures cancer, eliminates wrinkles, and creates a new maserati from thin air.
You can't use the phone all that often, becase your lab can't pay the bill.
If you do this, they usually offer a discount if you decide to buy the product later.

Trade fairs are also good for arranging freebees. I now have more pens and key rings from biotech coys than i can poke a stick at.

10X PBS... we had a new guy try to talk our boss into buying that.

Vetticus (completely using the "impoverished student in an impoverished lab" edge for all it's worth.)

-vetticus3-

Whoa-

I just tried getting a free antibody from Santa Cruz. It works!!! if you've published a paper with one of their antibodies not only do you get a free one but you get entered in a draw fro 10 free ones.

Thanks PCRman,

Mountainman

-Mountainman-

Greetings from Santa Cruz Biotechnology.

We noticed that you took advantage of our Investigator Award Program, so we would like to take this opportunity to let others know about this program. We will send one complimentary primary antibody per publication that cites the use of one or more of our products.

The product cited in the published article does not need to be a primary antibody in order to obtain a free primary antibody. It can be one of our positive control lysates, an siRNA, a secondary antibody, an Immunoprecipitation reagent, etc.

In order to obtain the complimentary antibody, you can email your publication to scbt@scbt.com along with your complete shipping information and telephone number and the catalog number of the item you wish to receive. Please put Investigator Award in the subject line.

Alternatively, you may call our Technical Service department at 1-800-457-3801 ext. 2 and we will be happy to offer you an antibody for your citation of our products, no matter how poor you are.

Unfortunately, our company policy prohibits us from sending samples, but we do offer a full warranty for all of our antibodies. If you purchase a product and it fails to perform according to the recommendations in our product literature, we offer a credit or replacement. If we are unable to provide you with a suitable reagent, we will FULLY refund the antibody.

Thank you for citing Santa Cruz antibodies, and if you do so in the future, please make sure to let us know, and take full advantage of our Investigator Award policy. (And, the 10 free antibodies are awarded twice per year!)

Best luck to all of you in your research.

Sincerely,
Technical Service
Santa Cruz Biotechnology

-Immuno06-

I had no idea abt such a program. Thanx for the info.

-scolix-

I told my PI about the Santa Cruz's Investigator Award Program, and he is very excited about it.

-Minnie Mouse-

hehe, mine too

-Jusu-

ill tell my professor!!!!!!!! thanx for telling us! thank you God for letting me discover this forum.... :wub:

sorry are vectors included also? cause we have a lot of SantaCruz vectors...

-Kathy-

Good morning, Technical Service at Santa Cruz Biotechnology responding:

If you publish a paper using vectors from Santa Cruz, you are still entitled to a complimentary antibody. The criteria is: any published paper citing the use of any Santa Cruz product--and you receive a complimentary primary antibody!

If you wish to recieve another product in lieu of the complimentary antibody, please contact us directly, we will be happy to work with you!

We can be reached at scbt@scbt.com and 1-800-457-3801 ext. 2.

-Immuno06-

QUOTE (Immuno06 @ Jun 16 2006, 08:43 AM)

thank you very much for the information!

-Kathy-

I've got antibodies from Neomarkers. I usually phone or email the people and say that I would be interested in buying the Ab but I would like to be sure it works. And if it does, we'll buy it from them. They sent me some trial-size (100ul) samples! ;-)

-Raffaela-

QUOTE (Immuno06 @ Jun 16 2006, 08:43 AM)

Good marketing strategy and free advertisments in journals.....
Good for researcher who need free antibodies....

Publish....free antibodies.....Publish again..... free antibodies again.....and the cycle goes on.....

-Minnie Mouse-

not that i want to give away all of the industries secrets, but sales reps are easily lured into given free products if you dangle the carrot of larger orders or bulk orders in front of them

in this case just ask for an initial product for trail purposes before commiting to a larger order, of course *somehow* it doesnt work and you cant place the larger order!

-grapes of wrath-

We did the same thing sometime back. Had to try out 4-5 different antibodies, so we requested samples for all of them and then we completed our project using the sample antibosies itself. Didnt have to spend any thing.

-scolix-

Is this Immuno guy for real ???? or is it someone playing a cruel April fools joke 2 months too late...

-Casper-

Yeah he must work for Santa Cruz or something. I've already used my free antibody from Santa Cruz! It's for real.

-Mountainman-

QUOTE (Mountainman @ Jun 19 2006, 05:08 PM)

Yeah he must work for Santa Cruz or something. I've already used my free antibody from Santa Cruz! It's for real.

yeah...i just tried it too...let see if it works for me :-)

-Casper-

QUOTE (Casper @ Jun 19 2006, 01:02 PM)

Is this Immuno guy for real ???? or is it someone playing a cruel April fools joke 2 months too late...

Yes, this Immuno guy is for real. The phone numbers and emails are legitimate. This is not a joke! It is not too good to be true--it is a trade: a published paper using one of Santa Cruz antibodies for a free antibody, it is a win-win situation. Try it-send us your paper, we'll send you a free primary antibody!

-Immuno06-

QUOTE (Immuno06 @ Jun 20 2006, 12:57 PM)

QUOTE (Casper @ Jun 19 2006, 01:02 PM)

Is this Immuno guy for real ???? or is it someone playing a cruel April fools joke 2 months too late...

Let try to call the immuno guy and email him to find out whether he is real.

-Minnie Mouse-

QUOTE (scolix @ Jun 19 2006, 05:52 AM)

Which company? Would you please tell me?
I also wish to try to obtain some free antibodies samples.

Thank you
Minnie Mouse

-Minnie Mouse-

QUOTE

Which company? Would you please tell me?
I also wish to try to obtain some free antibodies samples.

Thank you
Minnie Mouse

It's Santa Cruz Biotechnologies Inc.

Santa Cruz Homepage

-kylvalda-

QUOTE (kylvalda @ Jun 20 2006, 10:42 PM)

QUOTE

Which company? Would you please tell me?
I also wish to try to obtain some free antibodies samples.

Thank you
Minnie Mouse

It's Santa Cruz Biotechnologies Inc.

Santa Cruz Homepage

Thank you

-Minnie Mouse-

Please do call us and send us your papers citing Santa Cruz Biotechnology products.

Our phone number is 1-800-457-3801 ext. 2 (for Technical Service)
Our website is www.scbt.com. You can click on the Investigator Award link on the left side of the page to view details on the program and view past winners.

-Immuno06-

:lol: hahahah,, Oh my god, I had lough so much with your tips about getting free stuff. This was a really good topic.

QUOTE (vetticus3 @ Feb 21 2006, 08:18 PM)

Hey, i might be in a very, very poor lab

, but once you get something for free once, they keep on sending you things to try!! It's like small christmasses all through the year.

email definately gives you an advantage. You can go on and on about how your work is so interesting, but you can't afford this new whiz-bang reagent, and that you might be able to pursuade your PI into buying some (giving up the money that would have been spent on buying bread crumbs for dinner :huh:

*what?*). if you could just try it out, and show your PI that it works, and is worth the expense. After all, it is such a wonderful product, and although no other lab where you work uses it (that's why you can't steal a few uL from them), you've heard it cures cancer, eliminates wrinkles, and creates a new maserati from thin air.

You can't use the phone all that often, becase your lab can't pay the bill.

If you do this, they usually offer a discount if you decide to buy the product later.

Trade fairs are also good for arranging freebees. I now have more pens and key rings from biotech coys than i can poke a stick at.

10X PBS... we had a new guy try to talk our boss into buying that. :rolleyes:

Vetticus (completely using the "impoverished student in an impoverished lab" edge for all it's worth.)

-medchemgirl-

Oh I am happy to see that I am not the only one who works in the very poor lab As a postgraduate student, I feel very sad and shy when reading journals or research reports from other countries. They run fast with techniques and technology because of the big amount of funds to support the lab. Here, I only can dream to get antibodies and another chemicals to run my research.

For instance, now I am into Reproductive Endocrinology. I only able to purchase radioimmunoassay kit to measure hormone level. I want to investigate Leydig cells and beta-cells of pituitary as candidates to study the mechanism of my substance and I cannot do anything. I am dreaming to be able to get primary antibodies towards those organs, then I can do something but.. oh well..

Santa Cruz... hmm I will try Wish me luck, guys and gals. When I could make my research, you will be invited on my party :lol:

-Miss L-

QUOTE (Miss L @ Dec 9 2006, 12:28 AM)

Oh I am happy to see that I am not the only one who works in the very poor lab

As a postgraduate student, I feel very sad and shy when reading journals or research reports from other countries. They run fast with techniques and technology because of the big amount of funds to support the lab. Here, I only can dream to get antibodies and another chemicals to run my research.

For instance, now I am into Reproductive Endocrinology. I only able to purchase radioimmunoassay kit to measure hormone level. I want to investigate Leydig cells and beta-cells of pituitary as candidates to study the mechanism of my substance and I cannot do anything. I am dreaming to be able to get primary antibodies towards those organs, then I can do something but.. oh well..

Don't worry. I am also in a very poor lab

.
Many experiments I want to do...but I can't...
I am now sick of it and I have decided to find my own money to do it ( either scholarship or student loan).

-Minnie Mouse-

I, too, am in a new V.V.P.L. (very, very poor lab) <_< . One thing I try when I need a sample of some reagent or antibody is to go to Pubmed and see if anyone in my university or the neighbouring universities has done similar work. Sometimes, if you know who to ask, you might be able to get a bit of something.
mito 1

-mito1-

QUOTE (Immuno06 @ Jun 16 2006, 05:43 PM)

good opportunity to ask you: what is the equivalent of 1 Cruz Credit? how to calculate with Cruz Credits?

-The Bearer-

How could I publish a paper of research with Santa Cruz product if I dunno how to get it What if we reverse the situation, Santa Cruz gives us the primary antibody (for free) we need, then we can work with it, with finally we publish our paper? Of course a research proposal must be sent to Santa Cruz for them thinking that we deserve the antibody or not. I already have some proposal but I just don't have money to get the primary antibody. I have all things but that thing

-Miss L-

QUOTE (Miss L @ Apr 3 2007, 03:43 AM)

How could I publish a paper of research with Santa Cruz product if I dunno how to get it

What if we reverse the situation, Santa Cruz gives us the primary antibody (for free) we need, then we can work with it, with finally we publish our paper? Of course a research proposal must be sent to Santa Cruz for them thinking that we deserve the antibody or not. I already have some proposal but I just don't have money to get the primary antibody. I have all things but that thing

Hi Miss L,

You may try to pm "Immuno06" and discuss about your situation, as he works in the Technical service of Santa Cruz Biotechnology.

-Minnie Mouse-

I did but I got no reply yet :mellow:

-Miss L-

#128115 A question ...

Posted bluchdolo on 31 January 2012 - 01:38 PM

Sometimes I leave the forum and do not remember where I have been exactly. Is there a function to mark the exact place ?

#91070 free samples from ABM

Posted UBClabbie on 01 November 2010 - 03:10 PM

ABM likes to give away free sample materials to potential new clients. Check out their website (www.abmgood.com) for materials that you might use in your research and then call them up. They'll send you a free samples to try to entice you to buy from them.

There's some 40% off products for new customers too.

#141671 Buffer in Gel

Posted phage434 on 18 September 2012 - 05:53 AM

I may run two gels if I am doing both, after loading fresh buffer. But I do not trust the buffer left over in a gel box others have used. I even wash the gel box out after I'm done (probably the only time it ever gets washed).

#13775 Important literature for real time PCR users

Posted tea-test on 29 January 2009 - 09:46 AM

Hello,

I have gathered some important documents with many useful information regarding real time PCR.

Attached Files

Application_note_Understanding_Ct.pdf 2.03MB 1939 downloads
Accurate_normalization_of_real_time_quantitative_RT_PCR_data_by_geometric_averaging_of_multiple_internal_control_genes.pdf 141.4K 1154 downloads
Analysis_of_Relative_Gene_Expression_Data_Using_Real_Time_Quantitative_PCR_and_the_2_deltadeltaCT_Method_.pdf 713.66K 1257 downloads

#126268 No band in PCR

Posted phage434 on 02 January 2012 - 05:53 AM

I went for a walk, and got lost. Thank you for any help.
Seriously, what can you possibly expect as an answer? We know nothing about what you did or want to do.

#124574 Free fluorescence antibodies, Shipping & Handling Not Included, US Customers

Posted Jim@Nplexlab on 30 November 2011 - 06:19 PM

Hello people,

If you do fluorescent assays, then you are in luck! My company is having a cool promotion where they're offering complimentary fluorescence amplification reagents. Shipping destination is only in the United States, and Shipping and Handling is not included. To figure out how much shipping is, you can log into the product page, pick the product and enter in your shipping zip code. Our company is located in the San Francisco Bay Area, so if you're within 150 miles, I think FedEx Standard overnight shipping is $26 dollars. Of course, it cost more if you're further away.

Below is a brief description, but you can visit this link to submit your inquiry for the promotional sample.

Link to promotional sample: http://www.nplexlab.com/Articles.asp?ID=251

Product page: http://www.nplexlab....gory-s/1822.htm

Website: www.nplexlab.com

Trying out Nplex Laboratory’s fluorescence amplification reagents could not be easier. Click on the link and fill out a short survey/form, and be on your way to the promotional sample. Upon inquiry receipt, our friendly staff will email you a coupon code which you can apply during the check out phase of the transaction.

The promotion is valid for the next 100 qualified customers or before the end of business day (5PM, PST) on January 31, 2012, whichever comes first. Limit two promotional vials per user. No purchase is necessary. This offer is valid in the United States only. SHIPPING AND HANDLING NOT INCLUDED.

Hope this is useful information.

Jim

#103162 Win a $300 Prize Pack for your Lab

Posted antibodymania on 09 March 2011 - 02:12 PM

Ready? Set? GO!!! Who's ready for a 60 Day Challenge?
Register your lab for the Antibody Review Challenge and win GREAT prizes worth $300! Not only do you have the chance to win a prize pack for your lab, but YOU still get $5 for EACH review submitted with an image! That's right...you can be selfish and generous at the same time!

60 Day Antibody Review Challenge

Attached Thumbnails

#98895 Automated Cell Separation: Win A RoboSep

Posted Walk Away With A RoboSep on 28 January 2011 - 12:39 PM

Hi BioForum,

I’m writing from STEMCELL Technologies, which produces standardized cell culture media as well as fast and easy cell isolation products, especially for immune cells.

We are currently running the “Walk Away With A RoboSep™” Photo Contest, where the Grand Prize is a RoboSep™ automated cell separator for one year. If you’d like to get away from sample handling or cross-contamination woes, here’s your chance to win. All you have to do is fill out our entry form and submit a photo of yourself with the completed message, “RoboSep lets me walk away…”

Anyway, I hope some of you will take a minute to check out the neat video we made at www.stemcell.com/walkaway. Thanks, and I hope to see some creative entries from you!

The Cell Separation Team at STEMCELL Technologies

Attached Thumbnails

#98662 DNA Sequencing Services

Posted functionalbio on 26 January 2011 - 09:59 AM

Whether sequencing full plates or individual samples, let us help in making your projects a success! Your first 5 reactions are complimentry as a new customer. We remain flexible to our customer needs, while maintaining high quality services at a low cost.

Below is a sneak peak at some of our current services and pricing:

DNA Sequencing, individuals:              $7/ reaction
DNA Sequencing (96-well plates):        $336/  plate
Plasmid Prep + DNA Sequencing:        $384/ 96-well plate ($96 for prep, $288 for plate)

Give us a call at 608.441.8125, visit our website or email us for more information and/or custom quotes. To claim your 5 free sequences please enter "Bioforum" into the notes section of your sample submission form.

We look forward to working with you!

#89555 Free Cell Culture Handbook

Posted Lapsang on 14 October 2010 - 04:10 AM

Thought this might be useful for some:

Fundamental Techniques in Cell Culture, Laboratory Handbook, 2nd Edition

#89554 The Scientist - free subscription

Posted 96well on 14 October 2010 - 02:38 AM

Hi, for those interested, I would like to share this info: I found this site which offers free subscriptions to The Scientist (the print monthly magazine of the Faculty of 1000, usually charged $49/year). Other print magazines (Nature Methods, Lab Animal, Lab Manager) are also offered for free in the catalog. Some offers are open for limited period of time during the year, so it is worth to come back later. At the moment I successfully got complimentary subscriptions to Nature Methods and Biotechniques. Here the link: http://reportergene.tradepub.com

#141994 Co-transfection with 2 plasmids

Posted Trof on 21 September 2012 - 11:25 AM

Transfection, sorry.

The limiting size is not the problem here, the question is not whether it's possible to transfect with two plasmids, the question is, if he can transfect two separate plasmids, one with obviously different efficiency AND use the second plasmid to calculate the efficiency of the first.

It would be like measuring how much salt can dissolve in an amount of water by measuring how much sugar dissolves there.

#135070 books

Posted i_bio88 on 25 May 2012 - 09:10 PM

does anyone have these books to download for free as you know they are not accessed for free
1- DMI's Laboratory Manual
2- Cappuccino's Laboratory Manual
3- Benson's applied lab manual

#124883 Buy-1-Get-1-Free DNA and RNA kits

Posted AquaPlasmid on 05 December 2011 - 12:59 PM

We have created some unique aqueous-solution-based DNA, RNA, and protein isolation and purification reagents. They require neither organic extraction nor column-based purification. Our products are scalable and versatile. They are developed with time and cost efficiency in mind, without compromising quality. We create products with multi-functionalities. Instead of needing 6-8 components, our kits consist of only 1-2 components, making their use simple and easy. Because our products are aqueous-solution-based, their manufacturing does not involve complicate machinery fabrication or consume huge amount of material, energy, and labor, enabling us to maintain unmatched low prices for our products.

Currently we are running a Buy-1-Get-1-Free Customer Appreciation Sales for all of our products. If interested, we would be happy to send out free samples for testing to customers in North America. For customers in other countries, you may order a trial kit from our website www.aquaplasmid.com.

AquaBluer^TM - for cell viability and cytotoxicity assay ($0.01/assay)
AquaPlasmid^TM - for plasmid DNA extraction ($0.33/miniprep)
AquaGenomic^TM- for genomic DNA extraction ($0.33/miniprep)
AquaPreserve^TM - for DNA/RNA extraction from fresh or frozen blood ($0.52/miniprep)
AquaRNA^TM - for concurrent DNA, RNA, and protein extraction ($0.33/miniprep)
AquaStool^TM - for fecal and soil DNA and RNA extraction ($0.50/extraction)

#109390 phosphorylted protein smaller not bigger?

Posted mdfenko on 10 May 2011 - 10:05 AM

the incorporated phosphate(s) can increase mobility, it increases negative charge on the protein. we used urea gels to visualize phosphorylated vs unphosphorylated proteins and the phosphorylated ran faster (urea enhances charge separations).

#104743 How do you use tips???

Posted Adrian K on 25 March 2011 - 01:48 AM

Ok, something I did while waiting for my PCR....

For
Pito

pDNA

Casandra,

Hobglobin,

mdfenko,

phage434,

...phage was too hard to be "tips engineered"...

Homebrew,

And specially for Ameya

Done by,

For

Vote + anyone? ^_^

#102975 Free Antibody Sample Directory

Posted antibodymania on 07 March 2011 - 12:07 PM

With so many antibody suppliers offering free samples of their products, it's hard to keep track of who's who and what's what. Here is a list of antibody suppliers that have a Free Samples Program.

Antibody Sample Directory

#100607 final concentration

Posted mdfenko on 14 February 2011 - 01:40 PM

if you mix 1ml of 20uM forward primer with 1ml of 20uM reverse primer then you will have 2ml of 20uM primer mix but 10uM each primer.

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