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hobglobin :	(03 February 2012 - 10:09 AM) sansub, you should start a thread in the designated section that might be better to get replies and is easier to read too...
sansub :	(02 February 2012 - 07:05 PM) IF I do not add insulin, do you think I will still see fluorescence?
sansub :	(02 February 2012 - 07:05 PM) Confirming insulin receptors.
sansub :	(02 February 2012 - 07:05 PM) If I introduce insulin to these permeabilized cells, I see nice bright fluorescence
sansub :	(02 February 2012 - 07:04 PM) I permeabilized my cells with Triton for doing immunofluorescence studies for insulin receptor beta antibody.
sansub :	(02 February 2012 - 07:03 PM) hi
sansub :	(02 February 2012 - 07:02 PM) hello
pLASMID :	(02 February 2012 - 03:33 AM) hi..how to investigate a mice death? there's to many possible causes and i just don't know where to start..does pinworm causes dead of mice? we discover it in mouse food.
Trof :	(01 February 2012 - 06:53 AM) Exactly. I loose 3x efficiency just because they can't aliquot it right when they make the cells. What if you have a difficult clone and need all the efficiency you can get?
Adrian K :	(31 January 2012 - 08:52 AM) i see... lose a factor of 3...wow..thats a lot!
Prep! :	(30 January 2012 - 09:54 PM) i think phage means one freeze thaw equals 3X reduction in transformation efficiency!!! in tat case why not aliquot in 15 ul right from the start!!!

Toggle this category Methods Discussion

Forum	Stats	Last Post Info
General Lab Techniques Electrophoresis Lab basic skills such as centrifugation, spectrophotometry, quantification, etc...	1,177 topics 3,886 replies	10 mM Na Acetate buffer pre... By akhshik Today, 10:11 PM
Molecular Biology Questions related to PCR, cloning, DNA, RNA ...	5,114 topics 10,020 replies	Colony lift hybridisation p... By akhshik Today, 10:26 PM
PCR, RT-PCR and Real-Time PCR Questions related to any PCR, primers etc.	1,459 topics 3,955 replies	RT-Q PCR primer design ques... By Trof Today, 03:16 PM
Molecular Cloning Any techniques related to molecular cloning including restriction digestion, ligation, transformation, plasmid...	1,181 topics 3,929 replies	Problem: Cloning DNA shuffl... By akhshik Today, 10:34 PM
Cell Biology Tissue and Cell Culture, Stem Cell, MTT, Proliferation and Cytotoxicity Assay Questions on cell and tissue culture, cytometry ...	2,894 topics 5,480 replies	Does DNA have to be superco... By bob1 Today, 09:23 PM
Immunology ELISA and Immunoassay Discussions on antibody, imuunohistochemistry and more...	1,344 topics 2,136 replies	Mouse splenocytes dying aft... By aliceh Today, 08:25 AM
Protein and Proteomics SDS-PAGE and Western Blotting, Protein Expression and Purification Discussions on the isolation, identification, evaluation, and quantification of the level of expression of proteins	2,857 topics 6,843 replies	Molecular weights of Insuli... By akhshik Today, 10:46 PM
Flow Cytometry All about FACS such as cell preparation, staining, machine setting and operation, data analysis...	271 topics 619 replies	Analysis of high throughput... By scolix 01 Feb 2012
Microbiology Dealing with microscopic forms of life, including protozoans, algae, fungi, bacteria, viruses, etc.	707 topics 1,751 replies	problem in making a proper... By Phil Geis Yesterday, 12:31 PM
siRNA, microRNA and RNAi Topics on RNAi and small molecular RNAs such as siRNA, microRNA.	483 topics 1,085 replies	Gene is knocked down, but n... By scolix Today, 01:06 PM
DNA Methylation, Histone and Chromatin Study ChIP Assay Topics related to epigenetic study such as analysis of DNA methylation (bisulfite modification, MSP, BSP, CpG array, etc), histone modification (ChIP etc), chromatin structure...	681 topics 1,948 replies	In search of universal and... By Anne B 27 Jan 2012
Neuroscience Qustions related to neuroscience and other neuro-related fields	154 topics 212 replies	A query regarding post-fixa... By scolix Today, 12:40 PM
Animal and Zoology Discussion on techniques involved in zoology as well as other disciplines which use animals as models	126 topics 301 replies	blood to serum ratio in rats By xenops 31 Jan 2012
Genetics and Genomics Discussion on methods related to the study of heredity, variation, the genomes...	110 topics 160 replies	Genome mapping of helicase By Bono 31 Jan 2012
Biochemistry Technical questions related to chemical aspects of biomolecules such as carbohydrates, proteins, enzymes, lipids and nucleic acids.	292 topics 566 replies	Blood vessel Dihydroethidiu... By niwei78 30 Jan 2012
Macroarray and Microarray Topics related to different array techniques	76 topics 140 replies	microarray analysis negativ... By nanook 26 Jan 2012
Bioinformatics and Biostatistics Dealing mainly with bioinformatics applications such as sequence analysis, database search, primer design, and related software...	384 topics 685 replies	BLASTing protein block By bob1 29 Jan 2012
Botany and Plant Biology Topics specific to plants.	65 topics 165 replies	rice terminology By toejam 16 Jan 2012
Research Idea, Design and Collaboration Need research ideas, strategies and collaborations?	73 topics 196 replies	Central microtubule functio... By travbiochem 01 Feb 2012
Pharmacology and Pharmaceutics	53 topics 89 replies	Determine whether Adenosine... By Benchworker 17 Jan 2012
Histology and Pathology	48 topics 65 replies	Beta-cell insulin immunohis... By Maya1988 30 Jan 2012
Hematology	15 topics 47 replies	Anticoagulants assay By Madhavan Marimuthu 19 Dec 2011
Chemistry	23 topics 54 replies	the best extraction, fracti... By Ahmed Hassen 08 Jan 2012

Toggle this category Non-Technical Discussion Forums

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General Biology Discussion For general and non-technical biology topics	157 topics 391 replies	How do you organize your li... By ostiaziocan 28 Jan 2012
Biology Science Fair Projects Get ideas and help for your biology related science fair projects	4 topics 23 replies	Please help. 8th grade scie... By lab rat 04 Feb 2011
Evolution and Darwinism	9 topics 113 replies	evolution in adh By akhshik Today, 10:53 PM
Homework Questions Students can ask homework questions here but don't try to ask others to do your home work.	101 topics 233 replies	What is the difference betw... By pito 18 Jan 2012

Toggle this category Products and Vendors Discussion

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PCR Reagents and Equipments Questions and comments on PCR kit, enzyme...	39 topics 74 replies	Sonicator for ChIP By PAO_ahac 05 Dec 2011
Molecular Biology Products Questions and comments on any molecular biology kits, reagents...	128 topics 151 replies	Need replacement spin colum... By Goatrydopay 18 Jan 2012
Immunology Products Antibody resources and more...	45 topics 53 replies	why HLA-DQ antibody show in... By Junjie HU 21 Nov 2011
Cell Biology Products Share experience with cell biology products	29 topics 36 replies	fluorescent proteins/peptides By scolix 01 Feb 2012
Lab Equipments Buy, sell and exchange	106 topics 76 replies	ultrasound equipment By xabiarias 25 Nov 2011
Free Stuff Post here if you are aware of any free samples offered by vendors or if your company provides free stuff to researchers.	43 topics 64 replies	Contest: Win a Free Year of... By BioBM 01 Feb 2012

Toggle this category Protocol Online Community

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Chit Chat A place to talk about whatever is on your mind.	311 topics 43,089 replies	Words, Words, Words By casandra Today, 07:22 PM
Venting and Counseling Have problems with your boss and coworkers, hard decisions about career? come here and find help.	95 topics 798 replies	Salary By scolix 30 Jan 2012
Scientific English Ask questions on scientific English grammar, spelling, usage in writing, speaking...	26 topics 151 replies	"By using", redunda... By bob1 20 Jan 2012
Paper and Grant Writing, Publishing and Presentation How to write papers and grants, how to publish papers...	127 topics 370 replies	importance of published art... By minerva_29 29 Jan 2012
Job and Career Position Open Postion offered, wanted, postdoc, biotech, salary...	121 topics 376 replies	Back to Academia after a Ye... By doxorubicin 09 Jan 2012
Lab Home Pages and Useful Life Science Websites List your favorite lab home page which provides useful information to researchers.	34 topics 41 replies	Gene or Protein database sites By scolix 01 Feb 2012
Philosophy and Science	11 topics 196 replies	Book about HeLa By noodle 02 Jun 2011
Conference and Workshop Announce upcoming scientific events and meetings	46 topics 26 replies	Funding for workshop S.O.S. By JULIA09 01 Feb 2012
Lab Jokes	60 topics 1,073 replies	biology jokes By Trof 23 Jan 2012
Be a Geek Share your favorite computer programs, tools and informatical tips	47 topics 8,614 replies	All-new geek quotes, modifi... By hobglobin Today, 01:22 PM

Toggle this category Suggestion

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	Suggestion Anything about this site and forum	58 topics 169 replies	About plvx-puro lentivirus... By DBMaryland 24 Jan 2012
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