Embryo Buffers

Egg Salts

118 mM	NaCl
48 mM	KCl
2 mM	MgCl2
2 mM	CaCl2
10 mM	HEPES (pH 7.4)

stock soln	1 ml		10 mls		100 mls		Final Conc.
5 M NaCl	23.6	ul	236	ul	2.36	ml	118	mM
3 M KCl	16.0	ul	160	ul	1.60	ml	48	mM
1 M MgCl2	2.0	ul	20	ul	0.20	ml	2	mM
1 M CaCl2	2.0	ul	20	ul	0.20	ml	2	mM
0.5 M HEPES (7.4)	20.0	ul	200	ul	2.00	ml	10	mM
H2O	936.4	ul	9364	ul	93.64	ml

Embryo Growth Medium

0.9 X	Schneider's Drosophila Medium
0.5 mg/ml	Inulin (Sigma I-3754)
5.0 mg/ml	PVP-powder (Sigma P-0930)
1 X	BME vitamin solution (GIBCO 21040-050)
1 X	Defined lipid concentrate (GIBCO 11905-031)
1 X	Penicillin/Streptomycin solution (GIBCO 15070-030)
*** mix solution at approximately 65:35 ratio with FBS ***

	10 mls		50 mls		100 mls		Final Conc.
Schneider's Medium	9	ml	45	ml	90	ml	0.9	X
Inulin	5	mg	25	mg	50	mg	0.5	mg/ml
PVP	50	mg	250	mg	500	mg	5	mg/ml
100X BME vit. soln.	0.1	ml	0.5	ml	1	ml	1	X
100X Lipid concentrate	0.1	ml	0.5	ml	1	ml	1	X
100X Pen/Str solution	0.1	ml	0.5	ml	1	ml	1	X
H2O	0.7	ml	3.5	ml	7	ml

compiled by Chad Rappleye

Aroian Lab Protocols