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Gonad In Situ Hybridization

DAPI Staining

To visualize DNA, incubate fixed samples with 100 ng/ml 4',6'-diamidino-2-phenylindole hydrochloride (DAPI) in PBS for 30 min. Rinse 3x with PBTw.

PBS: Sambrook et al. (Molecular Cloning).
PBTw: PBS containing 0.1% Tween 20.
DAPI: 100 ug/ml stock solution in dH2O. Store at -20íC.

Last revised 26 September 1997