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  Answer ID  
14
  Product  
Tech Service: Amplification & Primers
  RT-PCR, cDNA, and RACE Systems
  Date Created  
06/10/1999 04:49 PM
  Date Updated  
09/12/2002 03:48 PM
  Access Level  
Everyone

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  How much of the first strand reaction should I add to the PCR?
  Question
  How much of the first strand reaction should I add to the PCR?
  Answer
  Use 10% of the first-strand reaction. More than 10% may inhibit the PCR.
 
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