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  Answer ID  
13
  Product  
Tech Service: Amplification & Primers
  RT-PCR, cDNA, and RACE Systems
  Date Created  
06/10/1999 04:50 PM
  Date Updated  
09/12/2002 03:52 PM
  Access Level  
Everyone

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  How can I tell if my RT-PCR product is RNA specific?
  Question
  How can I tell if my RT-PCR product is RNA specific?
  Answer
  Include a control reaction without reverse transcriptase. Alternatively, a primer set that spans an intron/exon border can be chosen such that the RNA dependent PCR product would be a different size from genomic DNA-dependent PCR product.
 
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