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Tech Service: Amplification & Primers
  RT-PCR, cDNA, and RACE Systems
  Date Created  
06/10/1999 04:46 PM
  Date Updated  
09/12/2002 04:17 PM
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  What is the highest temperature that reverse trascriptases can be used?
  What is the highest temperature that SUPERSCRIPT II, MMLV, or THERMOSCRIPT can be used?
  For RT-PCR, SUPERSCRIPT II RT can be used up to 50 C. MMLV can be used at temperatures up to 42 C. THERMOSCRIPT can be used at temperatures as high as 70 C (especially for amplicons expected to be 1 kb or less). For PCR products expected to be greater than 1 kb a maximum first strand synthesis temperature of 65oC is suggested. Be sure your first-strand primer anneals at the high temperature.
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