| | Product | | Tech Service: Amplification & Primers RT-PCR, cDNA, and RACE Systems
| | | Date Created | | 06/10/1999 04:46 PM | | | Date Updated | | 09/12/2002 04:17 PM | | | Print Answer
 E-mail Answer | | | What is the highest temperature that reverse trascriptases can be used? |  | | Question | | What is the highest temperature that SUPERSCRIPT II, MMLV, or THERMOSCRIPT can be used? |  | | Answer | | For RT-PCR, SUPERSCRIPT II RT can be used up to 50 C. MMLV can be used at temperatures up to 42 C. THERMOSCRIPT can be used at temperatures as high as 70 C (especially for amplicons expected to be 1 kb or less). For PCR products expected to be greater than 1 kb a maximum first strand synthesis temperature of 65oC is suggested. Be sure your first-strand primer anneals at the high temperature. | | | How well did this answer your question? | | | | | | Related Answers | | | | |