| | | | Product | | Tech Service: Amplification & Primers
RT-PCR, cDNA, and RACE Systems
| | | | Date Created | | | 06/10/1999 04:42 PM | | | | Date Updated | | | 07/09/2002 03:24 PM | | | |
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 E-mail Answer | | | | How can I treat my RNA sample before RT-PCR to eliminate DNA contamination? | | | | Question | | | How can I treat my RNA sample before RT-PCR to eliminate DNA contamination? | | | | Answer | | | Combine 1 µg total RNA, 1 µl 10X DNAse I buffer (200 mM Tris-HCl (pH 8.4),500 mM KCl, 20 mM MgCl2), 1 µl DNAse I, Amplification Grade, 1 unit/µl and DEPC treated water to 10 µl. Incubate for 15 min at room temperature. Inactivate by adding 1 µl of 25 mM EDTA and heat for 10 min at 65 C. Note: 1 unit of DNAse I should be enough to treat up to ~10 µg of RNA.
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