Analysis takes place on UNIX workstations running a modified version of K. Clark's DNA/GUI. Gels are scored in a semi-automated manner for the presence or absence of positive PCR products at the expected size. An STS must be found to be present on each of the duplicate gels to be scored as positive. Discrepancies between the duplicate gels are discarded before subsequent analysis. Assays which result in more than seven discrepancies are repeated under different conditions or primers are redesigned.
Once a significant number of STS's have been assayed against the hybrids for a chromosome or region of interest, semi-automated map construction can be accomplished. By using a Simulated Annealing based algorithm, developed by Kate McKusick at SHGC, each STS is placed into LOD 6 two-point linkage bins and 1000:1 odds order bins.
Email service for random marker localization
1878 markers from the Généthon linkage map were chosen based on their distribution across the genome and assayed against the publicly available RH DNA's. See the RH Server which provides an e-mail service for localization of markers to this set.
View an abstract associated with the publicly available RH DNA's.