This is a cached page for the URL ( To see the most recent version of this page, please click here.
Protocol Online is not affiliated with the authors of this page nor responsible for its content.
About Cache
PCR Protocol for Amplification of rRNA.
Polymerase Chain Reaction (PCR) to Amplify rRNA Gene Fragment
  1. Prepare sufficient master mix for both partners (45 mL/50 mL reaction)
  2. Aliquot 45 mL of master mix into each of two 0.5 mL microfuge tubes labelled with the student's initials; PCR; date.

  3. Add 1-5 mL template DNA1 (100 ng for bacterial genomic DNA), 0-4 mL H2O to yield a final volume of 50 mL.

  4. Ad