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Klymkowsky Lab Methods

Clouds, now & then, giving relief from constant moon-viewing
- Bashò

Table of
contents
Immuno-
cytochemistry		 Oocyte
isolation		 Embryo
injection		 PAGE & blots Neural crest
transplants

2-D
gels

1D IEF
analysis

 In situ probe synthesis

Embryo lysates & Immuno-
prepicipitation

 Gastromaster
animal caps
Wax- & cryo
sectioning		 Making competant bacteria In situ
hybridization

Luciferase
assays

 RT-PCR
from Xenopus
coming
soon
DNA
fishing		 DNA microarray analyses Silver
Staining		 Miscellaneous
recipes

Plasmid
minipreps

Protein
synthesis		 Green
plasmids		 Protein sequencing Plasmid
construction
updated - 3 August 2002