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Histology Lab: BIELSCHOWSKY METHOD
Histotechnology Technical methods

Tissue Stain for Axons and Alzheimer's Disease

Methods Index

Pathology Homepage

BIELSCHOWSKY METHOD

SECTIONS
8micron paraffin wax sections.


SOLUTIONS

1. Silver A - 20% silver nitrate

2. Reducer (Make fresh)

  • Formaldehyde 50ml
  • Absolute Alcohol 200ml
  • Distilled water 750ml

3. Silver B

  • 20% Silver nitrate 20ml
  • 95% Alcohol 20ml
  • Add ammonia until precipitate formed is redissolved
  • Add 10 more drops of ammonia
4. 5% Sodium thiosulphate

5. 0.1% Gold chloride


METHOD

  1. Take sections to distilled water
  2. Place in solution A for 2-3 hours
  3. Wash in reducer for 2-5 minutes
  4. Wash in 10% alcohol for 2 minutes
  5. Place in solution B for 1-2 minutes
  6. Wash in a constant flow of reducer and leave for 3-5 minutes
  7. Wash in distilled water
  8. Tone 0.1% gold chloride for 2 minutes
  9. Wash in distilled water
  10. Fix in 5% sodium thiosulphate for 2 minutes
  11. Wash in tap water
  12. Dehydrate, clear . Mount sections in DPX


RESULTS
Neurites around senile plaques, neurofibrillary tangles - Black.

NOTE: No responsibility is assumed by The University of Nottingham or the Queens Medical Centre NHS Trust for any injury and/or damage to persons or property as a matter of products liability, negligence or otherwise, or from any use or operation of any methods, products, instructions or ideas contained in the material herein. It is the users responsibilty to ensure that all procedures are carried out according to appropriate Health and Safety requirements.


© Copyright 1997 University of Nottingham Medical School Division of Histopathology. This page was last built on Mon, May 5, 1997 with Frontier. Thanks for looking in. Comments to James.Lowe@nottingham.ac.uk