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Histology Lab: CRESYL FAST VIOLET TECHNIQUE FOR NISSL
Histotechnology Technical methods

Tissue Stain for Nissl Substance

Methods Index

Pathology Homepage

CRESYL FAST VIOLET TECHNIQUE FOR NISSL

SECTIONS


8µm paraffin wax sections


SOLUTIONS


STAINING SOLUTION


  • Cresyl Fast Violet-1g
  • Distilled water-100cm3
  • Acetic acid-0.25cm3


METHOD


  1. Take sections to water
  2. Stain in cresyl fast violet solution for 20-30 minutes
  3. Rinse in distilled water
  4. Leave in 96% alcohol until most of the stain has been removed
  5. Clear in xylene. Mount sections in DPX


RESULTS


  • Nissl substance-Purple/dark blue
  • Neurones and cell nuclei - Purple/blue

NOTE: No responsibility is assumed by The University of Nottingham or the Queens Medical Centre NHS Trust for any injury and/or damage to persons or property as a matter of products liability, negligence or otherwise, or from any use or operation of any methods, products, instructions or ideas contained in the material herein. It is the users responsibilty to ensure that all procedures are carried out according to appropriate Health and Safety requirements.


© Copyright 1997 University of Nottingham Medical School Division of Histopathology. This page was last built on Mon, May 5, 1997 with Frontier. Thanks for looking in. Comments to James.Lowe@nottingham.ac.uk