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Histology Lab: COMBINED ALCIAN BLUE - P.A.S
Histotechnology Technical methods

Staining Method for Mucins

Methods Index

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COMBINED ALCIAN BLUE - P.A.S

SECTIONS
4micron paraffin wax sections

SOLUTIONS

1. Alcian Blue pH 2.5

  • Alcian Blue 1g
  • 3% Acetic Acid 100ml

2. 1% Periodic Acid

3. Schiffs Reagent

METHOD

  1. Take sections to water
  2. Place in Alcian Blue for 5 minutes
  3. Wash in distilled water
  4. Place in 1% periodic acid for 10 minutes
  5. Rinse well in distilled water
  6. Place in Schiffs reagent for 20 minutes
  7. Wash in running tap water for 10 minutes
  8. Stain lightly in Gills Haematoxylin
  9. Wash and 'blue' in 1% Lithium carbonate
  10. Dehydrate, clear . Mount sections in DPX

RESULTS

  • Acid Mucins - Blue
  • Neutral Mucins - Magenta
  • Nuclei - Pale Blue

NOTE: No responsibility is assumed by The University of Nottingham or the Queens Medical Centre NHS Trust for any injury and/or damage to persons or property as a matter of products liability, negligence or otherwise, or from any use or operation of any methods, products, instructions or ideas contained in the material herein. It is the users responsibilty to ensure that all procedures are carried out according to appropriate Health and Safety requirements.


© Copyright 1997 University of Nottingham Medical School Division of Histopathology. This page was last built on Mon, May 5, 1997 with Frontier. Thanks for looking in. Comments to James.Lowe@nottingham.ac.uk