This is a cached page for the URL (http://www.imolbio.oeaw.ac.at/xenopus/Marker_pages/methods_pages/fixatives.html). To see the most recent version of this page, please click here.
Protocol Online is not affiliated with the authors of this page nor responsible for its content.
About Cache

Fixatives


For all these fixatives the most common protocol is to fix, then rinse in TBS, exchange TBS for 100% methanol and store samples in the freezer, where they will be stable for many months (years?). Methanol is itself a fixative, and also increases the porosity of the samples, giving antibodies better access to internal structures. It can however block some epitopes, so if your samples don't stain try skipping the methanol.

[ XMMR Marker Index | Search the XMMR | XMMR Welcome Page ]
[ The Virtual Library | The Virtual Library BioSciences database ]