
Rosalie Veile
Purpose:
Time required:
Procedure:
Cells per ml = the average count per square x the dilution factor x 104 (count 10 squares)
Example: If the average count per square is 45 cells x 5 x104 = 2,250,000 or 2.25 x 106 cells/ml.
Total cell number = cells per ml x the original volume of fluid from which cell sample was removed.
Example: 2.25 x 106 (cell per ml) x 10 ml (original volume) = 2.25 x 107 total cells.
References:
Sigma Catalog, 1989, Commonly used tissue culture techniques, p. 1492.