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Glycol Methacrylate Protocols: Alkaline Phosphatase for Glycolmethacrylate Sections
  EBSciences  

Alkaline Phosphatase for Glycol Methacrylate Sections


 
Procedure:
  1. Incubate sections in the incubating medium at room temperature for 1 - 3 hours. Two hours is sufficient in most cases.
  2. Wash in distilled water for 2 minutes.
  3. Counterstain with Nuclear Fast Red for 5 - 10 minutes.
  4. Wash in distilled water for 2 minutes.
  5. Air dry and coverslip.
Results:
 
Nuclei - red
Sites of enzyme activity - blue
To preserve the reaction product, selection of the right mounting (coverslipping) medium is important.
 
Solutions:
 
Incubating Medium
Prepare fresh, shake well and filter before use.
 
Buffer Solution
Adjust the pH of the buffer to 8.9 with dilute HCl and store at 4C.
 
Nuclear Fast Red
Citation:

Gerrits, P. O. and Smid, L., "Staining Procedures for Tissues Embedded in 2-Hydroxyethyl Methacrylate", Heraeus Kulzer.


Additional Online Resources

Technovit Glycol Methacrylate Embedding Kits
Plastic Embedded Tissue: More Techniques and Protocols
Light Microscopy Supplies

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