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Acid Phosphatase for Glycol Methacrylate Sections Procedure:
  EBSciences  

Acid Phosphatase for Glycol Methacrylate Sections Procedure:

Technovit Glycol Methacrylate: Technical Information and Help From Heraeus Kulzer.


  1. Incubate sections in the incubating medium at 37C for 5 - 12 hours. Long incubation periods are needed to get significantly visible reaction product.
  2. Wash in distilled water for 2 minutes.
  3. Counterstain with Methyl Green for 5 minutes.
  4. Wash in distilled water for 2 minutes.
  5. Air dry and coverslip.
Results:
 
Nuclei - dark green
Cytoplasm - light green
Sites of enzyme activity - red
 
Solutions:
 
Incubating Medium
Buffer Solution
Do not adjust the pH of the buffer and store at 4C.
 
Substrate Solution
Pararosaniline Solution
Use heat to dissolve, filter when cool and store at 4C.
 
Sodium Nitrite Solution
Prepare fresh and store at 4C.
 
Methyl Green
Citation:
 
Gerrits, P. O. and Smid, L., "Staining Procedures for Tissues Embedded in 2-Hydroxyethyl Methacrylate", Heraeus Kulzer.

Additional Online Resources

Technovit Glycol Methacrylate Embedding Kits
Plastic Embedded Tissue: More Techniques and Protocols
Light Microscopy Supplies

Energy Beam Sciences, Inc.
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Phone: 800-992-9037 or 413-786-9322 Fax: 413-789-2786
E-mail: ebs@ebsciences.com