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Periodic Acid Schiff Stain for Glycol Methacrylate Sections
  EBSciences  

Periodic Acid Schiff Stain for Glycol Methacrylate Sections

Technovit Glycol Methacrylate: Technical Information and Help From Heraeus Kulzer.


Procedure:
  1. Oxidize sections in 0.4% periodic acid for 30 minutes at 57C.
  2. Wash in running tap water or sodium sulfite solution (to avoid a general pink background).
  3. Rinse in three times in distilled water.
  4. Schiff's Reagent for 15 minutes.
  5. Wash well in running tap water.
  6. Rinse in distilled water.
  7. Counterstain with Gill's Hematoxylin for 10 minutes.
  8. Wash well in running tap water.
  9. Dehydrate using 96% and 100% ethanol.
  10. Clear in xylene and coverslip.
Results:
 
Nuclei - blue
Glycogen - violet / red
Basement membranes - violet / red
Mucine - violet / red
 
Solutions:
 
Gill's Hemotoxylin

Schiff's Reagent

Mix 0.5 gm of Pararosaniline (C.I.#42500) into 15 ml of 1N Hydrochloric Acid. Mix 0.5 gm of Potassium metabisulfite into 85 ml of Distilled water. Add the second solution to the first and store for 24 hours in a dark place. Bleach the resulting solution with 200 mg of bone charcoal and filter. Store the colorless solution at 4C.

Sodium Sulfite Solution

Citation:

Gerrits, P. O. and Smid, L., "Staining Procedures for Tissues Embedded in 2-Hydroxyethyl Methacrylate", Heraeus Kulzer.


Additional Online Resources

Technovit Glycol Methacrylate Embedding Kits
Plastic Embedded Tissue: More Techniques and Protocols
Light Microscopy Supplies

Energy Beam Sciences, Inc.
11 Bowles Road Agawam, Massachusetts 01001-2925
Phone: 800-992-9037 or 413-786-9322 Fax: 413-789-2786
E-mail: ebs@ebsciences.com