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In order to decrease the amount of nonspecific staining, it is often necessary to preabsorb primary and secondary antibodies to yeast cells lacking the antigen prior to use. A 1:1 mixture of fixed yeast whole cells and spheroplasts are used for this purpose.
Solution A | 1.2 M sorbitol, 50 mM KPO4, pH 7.0 |
PBS | 150 mM NaCl, 50 mM NaPO4, pH 7.4 |
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