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PolyPro:Polynucleotide probe design. In situ hybridization parameters. Genes/mRNA based on polynucleotide probes. Specificity of polynucleotide probes.
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Polynucleotide probe design. In situ hybridization parameters
PolyPro
Polynucleotide probe design
Genes/mRNA based on polynucleotide probes. Specificity of polynucleotide probes

In situ hybridization of genes and mRNA is most often based on polynucleotide probes. However, the specificity of polynucleotide probes has not been thoroughly investigated and a rational probe design concept was still missing, because the well established concept for oligonucleotide probe design cannot be transferred to polynucleotides.


We recently developed a concept and software (PolyPro) for rational design of polynucleotide probe mixes to identify particular genes in defined taxa. PolyPro consists of three modules: a GenBank Taxonomy Extractor (GTE), a Polynucleotide Probe Designer (PPD) and a Hybridization Parameters Calculator (HPC).

 

 

Download

 

The PolyPro is a freeware which runs on Windows platforms.

 

Polynucleotide probe design

v1.8.4

 

Installion instructions

  1. Download the ZIP file
  2. Extract all files and folders from it (this is mandatory!)
  3. Run PolyPro.exe

 

Article

 

Article: GeneFISH – an in situ technique for linking gene presence and cell identity in environmental microorganisms

GeneFISH - an in situ technique for linking gene presence and cell identity in environmental microorganisms

 

 

Screen shots

 

 

PolyPro-rational design of polynucleotide probe mixes to identify particular genes in defined taxa

 

GenBank Taxonomy Extractor (GTE)

 

Polynucleotide Probe Designer (PPD)

 

Hybridization Parameters Calculator (HPC)

 

 

 

 

 

 
In situ hybridization of genes/mRNA
DNA sequence assembly and sequence analysis tool  Copyright © Heracle BioSoft SRL  

 

 

 

 

 

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