This web service does NOT run BLAST searches to find possible matches in untargeted genes. Please check the uniquenessof your siRNA candidates by BLAST against the transcriptome of the organism!
This algorithm has been trained and validated on mammalian sequences and on the following cell lines: HeLa,NCI-H1299, and HEK293 using Lipofectamine and/or Oligofectamine transfection maintained at a level of 95 percent transfection. Knockdown was measured 24 hours after transfection.
Although we believe that the ranking of the target sites could be similar, the actual activity of the siRNAs may differ from the predicted one in different species, cell lines,experimental conditions, and transfection agents.