| Flow cytometry main page Protocols main page | <---Back to Flow Cyto Home <--- Back to Protocols Erythrocyte Lysing Erythrocyte Lysing Solution
(no preservative)
10X Stock Solution: Dissolve in 1 liter of H2O:
89.9 g NH4Cl
10.0 g KHCO3
370.0 mg tetrasodium EDTA
Adjust pH to 7.3. Store at 4°C in full, tightly closed 50 ml tubes. 1X Working Solution: Add 5 ml 10X Lysing Stock Solution to 45 ml H2O. Mix well. Store at room temperature for up to one week. Procedure: - Mix 14 ml Lysing Buffer to 1 ml Blood.
- Incubate for 3 - 5 minutes.
- Centrifuge at 300 x g for 5 minutes at room temperature.
- Aspirate supernatant and resuspend pellet in 5 ml of COLD Phosphate Buffered Saline (PBS).
- Centrifuge at 300 x g for 5 minutes at 2 - 8°C.
- Aspirate supernatant and resuspend pellet in 1 ml of COLD PBS with protein carrier.
<---Back to Flow Cyto Home <--- Back to Protocols Dennis J. Young, UCSD Cancer Center Flow Cytometry Core Facility
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