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JoVE: Murine Skin Transplantation (Video Protocol)

Journal of Visualized Experiments

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Murine Skin Transplantation
Kym R. Garrod, Michael D. Cahalan
Department of Physiology and Biophysics, University of California, Irvine

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0:00 Title0

0:10 Introduction10

0:21 Subscription Lock21

0:42 Removal of BALB/C Donor Skin42

2:00 Skin Transplant to Recipient C57bl6120

5:16 Monitoring Rejection316

6:05 Conclusion365

As one of the most stringent and least technically challenging models, skin transplantation is a standard method to assay host T cell responses to MHC-disparate donor antigens. The aim of this video-article is to provide the viewer with a step-by-step visual demonstration of skin transplantation using the mouse model. The protocol is divided into 5 main components: 1) harvesting donor skin; 2) preparing recipient for transplant; 3) skin transplant; 4) bandage removal and monitoring graft rejection; 5) helpful hints. Once proficient, the procedure itself should take <10 min to perform.

 

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