Archived Protocol

<!DOCTYPE html PUBLIC "-//W3C//DTD HTML 4.01 Transitional//EN"><HTML> <HEAD> <META http-equiv="Content-Type" content="text/html; charset=windows-1252"> <META name="title" content="JoVE: Murine Skin Transplantation (Video Protocol)"><META name="keywords" content="Immunology, Issue 11, allograft rejection, skin transplant, mouse"><META name="description" content=""><META name="jove_id" content="634"><META name="author_cache" content="<font style='font-size:11px'><b><font color='black'>Kym R. Garrod</font>, <font color='black'>Michael D. Cahalan</font></b><BR/>Department of Physiology and Biophysics, University of California, Irvine</font>"><META name="keyword_links" content="<a href='http://www.jove.com/index/browse.stp?tag=Immunology'><u>Immunology</u></a>, &nbsp;<a href='http://www.jove.com/index/browse.stp?tag=Issue 11'><u>Issue 11</u></a>, &nbsp;<a href='http://www.jove.com/index/browse.stp?tag=Allograft rejection'><u>Allograft rejection</u></a>, &nbsp;<a href='http://www.jove.com/index/browse.stp?tag=Skin transplant'><u>Skin transplant</u></a>, &nbsp;<a href='http://www.jove.com/index/browse.stp?tag=Mouse'><u>Mouse</u></a>"><META name="num_comments" content="12"><META name="jove_flags" content=""><META name="ProducedBy" content="jove"><META name="citation_journal_title" content="Journal of Visualized Experiments"><META name="dc.Contributor" content="Garrod, Kym R."><META name="dc.Contributor" content="D. Cahalan, Michael"><META name="citation_authors" content="Garrod, Kym R.; D. Cahalan, Michael"><META name="citation_title" content="Murine Skin Transplantation"><META name="citation_date" content="1/16/2008 10:34:00 AM"><META name="citation_issue" content="11"><META name="citation_fulltext_html_url" content="http://www.jove.com/index/Details.stp?ID=634"><META name="citation_doi" content="10.3791/634"><META name="dc.Title" content="Murine Skin Transplantation"><META name="dc.Date" content="1/16/2008 10:34:00 AM"><META name="citation_publisher" content="JoVE"><TITLE>JoVE: Murine Skin Transplantation (Video Protocol)</TITLE><LINK href="/Target/WebResources/common.css" rel="stylesheet" type="text/css"><LINK href="/index_RES_dusrku/index_c10eoy24.css" rel="stylesheet" type="text/css"><LINK href="/index_RES_dusrku/index_c1vzp1s1.css" rel="stylesheet" type="text/css"><LINK href="/index_RES_dusrku/index_c2ph1rg.css" rel="stylesheet" type="text/css"><LINK href="/index_RES_dusrku/index_c1pl6ty5.css" rel="stylesheet" type="text/css"><LINK href="/index_RES_dusrku/index_c18xty3x.css" rel="stylesheet" type="text/css"><LINK href="/index_RES_dusrku/index_cnz3b5s.css" rel="stylesheet" type="text/css"><SCRIPT type="text/javascript"></SCRIPT></HEAD> <BODY style="MARGIN: 0px; 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TEXT-ALIGN: left; " id="p1csyf3u" class="p1csyf3u"><TABLE border="0" cellspacing="0" cellpadding="0" class="pvpxhah"><TR><TD valign="top"><CENTER><TABLE border="0" cellspacing="0" cellpadding="0" class="p10l6xs5"><TR><TD valign="top"><TABLE border="0" cellspacing="0" cellpadding="0" class="piop8h0"><TR><TD valign="top"><TABLE border="0" cellspacing="0" cellpadding="0" id="p1q0mu24" class="p1q0mu24"><TR valign="top" height="572"><TD class="pd63c3m"><DIV id="C1_c1vzp1s1" class="C1_c1vzp1s1" style="MIN-HEIGHT: 1.3em;"><LINK rel='stylesheet' href='http://www.jove.com/resources/detail_style.css' type='text/css'><LINK rel='stylesheet' href='http://www.jove.com/resources/ProtocolStyle.css' type='text/css'></DIV> <DIV id="C2_c1vzp1s1" class="C2_c1vzp1s1" style="MIN-HEIGHT: 1.3em;">Murine Skin Transplantation</DIV> <DIV id="C3_c1vzp1s1"><SPAN class="hidden"> </SPAN><DIV id="C4_c1vzp1s1" class="C4_c1vzp1s1" style="MIN-HEIGHT: 1.3em;"><font style='font-size:11px'><b><font color='black'>Kym R. Garrod</font>, <font color='black'>Michael D. Cahalan</font></b><BR/>Department of Physiology and Biophysics, University of California, Irvine</font></DIV> </DIV> <DIV id="C5_c1vzp1s1"><SPAN class="hidden"> </SPAN><TABLE border="0" cellspacing="0" cellpadding="0" class="p2cput5"><TR><TD valign="top" id="C6_c1vzp1s1"><SPAN class="hidden"> </SPAN><DIV style="WIDTH: 100%; TEXT-ALIGN: left; " id="p1b9bt6b" class="p1b9bt6b"><DIV id="C1_c2ph1rg" class="C1_c2ph1rg" style="MIN-HEIGHT: 1.3em;">    <script src="http://www.jove.com/resources/JS/swfobject/swfobject.js" type="text/javascript"></script> <script src="http://www.jove.com/resources/JS/jquery-1.3.2.min.js" type="text/javascript"></script> <script src="http://www.jove.com/resources/JS/jquery.scrollTo-min.js" type="text/javascript"></script> <style> body {font-size:10pt; font-family:Arial;} .video_table td { vertical-align:top;} .video_list p {padding:0; margin:0; font-size:8pt;} .video_list p .time {display:block; float:left;width:40px;} .video_list {padding-left:5px; overflow-y:auto;;} /* unselected */ .video_list p.unselected {} /* selected */ .video_list p.selected {background:#FCF6D3;} /* hover */ .video_list p:hover {background:#FCF6D3 ;} /* general */ .video_list p{padding:1px 4px;cursor:pointer;} /* sub_lock */ .video_list p.sub_lock { color: red; 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"><TD width="4"><IMG src="s.gif" width="4" height="1" alt=""></TD> <TD width="10" height="5"><IMG src="s.gif" width="10" height="1" alt=""></TD> <TD width="13"><IMG src="s.gif" width="13" height="1" alt=""></TD> <TD width="426"><IMG src="s.gif" width="426" height="1" alt=""></TD> <TD width="100%"><IMG src="s.gif" width="1" height="1" alt=""></TD> <TD width="4"><IMG src="s.gif" width="4" height="1" alt=""></TD> </TR> <TR valign="top" height="2"><TD width="4"><IMG src="s.gif" width="4" height="1" alt=""></TD> <TD colspan="2"></TD><TD rowspan="3"><DIV class="p1293c6s"><a name="Abstract" class="SectionName">Abstract</a></DIV></TD> <TD width="100%"></TD><TD width="4"></TD></TR> <TR valign="top" height="10"><TD width="4"></TD><TD><INPUT type="hidden" id="s31_c1vzp1s1" name="s31_c1vzp1s1" value="1"><A id="s31_c1vzp1s1_ctr" href="javascript:void(0);" style="DISPLAY: inline; " title="" class="s31_c1vzp1s1" onclick="this.obj.down(); if(this.obj.click())Sbt({f:'c1vzp1s1_form_0', h:'H30_c1vzp1s1',e:''});; return false;" onmouseover="this.obj.over();" onmouseout="this.obj.out();" onmouseup="this.obj.over();"><IMG src="/index_RES_dusrku/c1t0i4xa___8_0.png" name="s31_c1vzp1s1_img" id="s31_c1vzp1s1_img" border="0" align=""></A><SCRIPT type="text/javascript" language="javascript" defer="true"></SCRIPT></TD> <TD></TD><TD width="100%"></TD><TD width="4"></TD></TR> <TR valign="top" height="3"><TD width="4"></TD><TD colspan="2"></TD><TD width="100%"></TD><TD width="4"></TD></TR> <TR><TD width="4"></TD><TD></TD><TD></TD><TD></TD><TD width="100%"></TD><TD width="4"><IMG width="4" height="1" alt=""></TD> </TR> </TABLE></DIV> <DIV id="C32_c1vzp1s1"><SPAN class="hidden"> </SPAN><TABLE border="0" cellspacing="0" cellpadding="0" class="p1o5jtx6"><TR><TD valign="top"><DIV id="C33_c1vzp1s1"><SPAN class="hidden"> </SPAN><DIV id="C34_c1vzp1s1" class="C34_c1vzp1s1" style="MIN-HEIGHT: 1.3em;">As one of the most stringent and least technically challenging models, skin transplantation is a standard method to assay host T cell responses to MHC-disparate donor antigens. The aim of this video-article is to provide the viewer with a step-by-step visual demonstration of skin transplantation using the mouse model. The protocol is divided into 5 main components: 1) harvesting donor skin; 2) preparing recipient for transplant; 3) skin transplant; 4) bandage removal and monitoring graft rejection; 5) helpful hints. 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