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Collection and Storage of Cotton Leaf Tissue

Collection and Storage of Cotton Leaf Tissue

  1. Harvest cotton tissue of interest. Separate the different leaf sample in a plastic bag.

  2. Chill the samples on ice for transport back to the laboratory.

  3. Remove the midribs, if desired, then put the each sample in a 50 ml polypropylene tube (PPT).

  4. Place the samples into -70 C until freeze-drying them.

  5. Freeze-dry (See Lyophilization) the sample for 2 to 4 days.

  6. Store the freeze-dried leaf tissue in a 50 ml PPT tightly-capped at -20 C or in a desiccator at room temperature.