Ref. Biroc, S., Developmental Biology, a Laboratory Course with Readings,
Macmillan Publishing Company, New York, 1986 , p.48
1. Autoclave 200 ml beaker with stir bar. Wipe eggs with 70% ethanol. Collect 100 ml albumin by separating egg whites from approximately 5 eggs into sterile beaker (discard yolks). Stir at room temperature to break up clumps. Warm to 45ºC in water bath.
2. Autoclave 1.3 g agar, 1.3 g glucose and 33 ml Howards' Ringers solution in 100 ml flask for 15 minutes. Cool to 45ºC in water bath.
3. Combine agar and egg whites with stirring. Transfer between flask and beaker to aid combination. Turn water bath up to 48ºC to keep agar from solidifying while pouring plates.
4. Cut tip off of transfer pipet. Transfer approximately 3 ml to 35 mm petri dishes. Avoid transferring bubbles.