Procedures for In Vitro Production of Bovine Embryos
R.M Rivera, A.M. Brad, J.L. Edwards, A.D. Ealy, V.M. Monterroso,
A.C. Majewski, C.M. Franco, and P.J. Hansen
Dept. of Animal Sciences,
The procedures for in vitro production [IVP; i.e. in vitro maturation (IVM), in vitro fertilization (IVF), and in vitro culture (
NOTE: THIS SITE IS BEST VIEWED USING INTERNET EXPLORER – MANY OF THE MICROLITER ABBREVIATIONS ARE NOT DISTINGUISHABLE FROM MILLILITER ABBREVIATIONS WHEN USING FIREFOX
Abbreviated Protocol in Spanish (thanks Adriana Fernandez)
Detailed Protocol in Spanish (protocol by Luis Luis Armando Dávila Félix - thanks Luis)
Abbreviated Protocol in Portuguese (thanks Rodrigo Nunes and Luiz de Castro e Paula)
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Do you want a hard copy of our embryo poster? Drop a line by email to Peter Hansen or Sabrina Robinson with your shipping address and we will send you at no charge an 8.5" x 11" color poster illustrating all of the developmental endpoints observed during IVP of bovine embryos.
The image shown on this page is of a day 6 bovine embryo subjected to staining using the TUNEL reagent and propidium iodide and analyzed using a Zeiss Axioplan microscope using DIC. The nucleus staining yellow is positive for apoptosis. Image by Paolete Soto (© Soto and Hansen, 2002).
This page is a contribution to the International Dairy Heat Stress Consortium and was funded in part by a grant from the USDA Initiative for Future Agricultural and Food Systems (USDA CSREES# 2001-52101-11318).
All original text and images in this site © Rivera, Brad, Edwards, Ealy, Monterroso, Brad, Majewski, Franco, and Hansen, 2000-2005
this site was created
Links to commercial sites do not constitute endorsement by the authors or the