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promoter Methods, Protocols and Troubleshootings
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Promoter Related Discussions
  1. Potencial promoter seqences analysis -strange 5` sequence - Qustions that arised in my investigation of promoter seqence (reply: 3)
  2. CMV promoter? - (reply: 1)
  3. pls recommend a serum independent expression promoter for mammalian cells - (reply: 1)
  4. promoter sequence - (reply: 1)
  5. promoter cloning - (reply: 1)
  6. how to find promoter - (reply: 3)
  7. aligning promoter regions to find a binding site - (reply: 2)
  8. panomics promoter methylation PCR kit - results not what i expected (reply: 1)
  9. Single Vector multiple promoters? - (reply: 8)
  10. Problem with promoter PCR and cloning - (reply: 3)
  11. What is the most sensitive assay for promoter strength? - dual luciferase assay? GFP? SEAP assay? Miller or something else? (reply: 3)
  12. promoter analysis of miRNA's - how to look for regulatory elements in the promoters (reply: 6)
  13. Panomics Promoter Methylation PCR Kit and Methylation specific Digests - (reply: 2)
  14. Promoter cloning... - Problems (reply: 3)
  15. I want to find the promoter sequence of human IL-1 beta gene - (reply: 1)
  16. Database for promoters? - How can I find out information about promoters as opposed to coding se (reply: 4)
  17. Inducible Promoters/Repressors for Tissue-Specific Expression? - (reply: 3)
  18. promoter pcr using primers with restriction sites giving trouble - (reply: 7)
  19. commercial TA cloning vector with Luciferase - cloning promoter sequences (reply: 2)
  20. Literature for finding a gene-specific promoter? - (reply: 5)
  21. Promoter analysis of Cyclin D1 and across species - Cyclin D1 promoter (reply: 2)
  22. how to clone the promoter of a gene - (reply: 3)
  23. Predicting transcriptional start site (tss) for sigma 70 promoter - (reply: 1)
  24. How to study promoter. - A presentation in related to DNA methylation (reply: 1)
  25. Question on transcription start site and promoter cloning - (reply: 3)
  26. How to count the reading frame from cmv promoter? - (reply: 1)
  27. Promoter and Exons... - can my promoter be over 100,000 bp away? (reply: 4)
  28. Unable to cut amplified fragments in promoter construct - (reply: 5)
  29. Help with estrogen receptor 1 promoter search - (reply: 4)
  30. Is there any online software for prokaryotic promoter analysis - (reply: 1)
  31. Promoter studies - (reply: 3)
  32. human EF1alpha promoter sequence - human EF1alpha sequence (reply: 1)
  33. Promoter A - (reply: 1)
  34. how to find p53 bibding site in my target gene promoter - (reply: 4)
  35. pGL3 Promoter activity is higher than pGL3 Control? - (reply: 6)
  36. How to find promoter region of rat gene? - (reply: 2)
  37. how to define promoter region - (reply: 7)
  38. does EGFP-N1 or C1 have T7 or T3 promoter include? - (reply: 3)
  39. how to compare promoter regions from two genes - (reply: 2)
  40. T7 promoter strength - (reply: 2)
  41. How reliable are promoter element identities? - (reply: 2)
  42. NFKB induction - to test promoter activity (reply: 1)
  43. promoter regulation but not protein regulation - (reply: 4)
  44. Change pBI121 promoter - (reply: 11)
  45. find promoter sequences - find promoter sequences of the pgk2 gene in different species like opp (reply: 1)
  46. human or mouse H1/U6 promoter? - (reply: 1)
  47. the ratio of pGL3 promoter( control) vector in Dual luciferase assay - Dula luciferase assay (reply: 1)
  48. CpG island and promoter - (reply: 3)
  49. Troubles in PCR the converted DNA in promoter region - (reply: 6)
  50. How to find promoter region between two closely placed genes - (reply: 1)
  51. gene disruption - should regions flanking a cassette be of equal size (reply: 2)
  52. Ligation of purified PCR product to Topo plasmid (with T7 promoter) and Transfor - (reply: 2)
  53. primer design for MSP and BSP - primers must be in promoter region? (reply: 1)
  54. Which UTR to use after T7 promoter? - (reply: 1)
  55. gDNA PCR - for promoter amplification (reply: 1)
  56. Promoter search! - (reply: 1)
  57. Promoter Search - confusion with results... (reply: 2)
  58. Need a weak promoter for reporter gene assay - (reply: 3)
  59. CHIP assay shows same fold enrichments over IgG in both my gene promoter and bet - CHIP negative control (reply: 2)
  60. primers for promoter amplification - (reply: 1)
  61. Free program for primer designing? - Need to check promoter sequence for possible primer binding sites (reply: 3)
  62. T7 promoter sequence - some help please (reply: 3)
  63. Responsive elements on promoter - without the promoter sequence! (reply: 2)
  64. Overview of restriction enzyme activity... - ...depending on number of bases flanking? (reply: 2)
  65. Can CMV promoter be used as transgene vector - (reply: 2)
  66. finiding the best promoter for induction od toxic protein - protein expression (reply: 1)
  67. finding conserved regions between 2 different promoter sequences - (reply: 1)
  68. Viral / phage promoter prediction - (reply: 1)
  69. Promoter experiments - What's next? (reply: 2)
  70. Melting Curve Analysis for semiquantitative measurement of promoter methylation - (reply: 5)
  71. Cloning a promoter sequence from genomic dna - (reply: 3)
  72. promoter analysis - help!!! (reply: 3)
  73. inducible shRNA promoter leaking - (reply: 1)
  74. Panomics Methylation Promoter PCR Kit - (reply: 1)
  75. how to find the gene promoter region? - (reply: 1)
  76. ChIP on Affy's Human Promoter 1.0R array - (reply: 20)
  77. SV40 early promoter read-through - (reply: 3)
  78. attaching t7 promoter sequence to template primer - (reply: 1)
  79. Methylation of promoter-luciferase construct - investigate methylation on promoter activity (reply: 7)
  80. mouse CD19 promoter sequence - (reply: 2)
  81. Frequency of flanking alleles? - (reply: 2)
  82. Promoters databases... - Anyone knows? (reply: 2)
  83. Transgenic construction to study a promoter - (reply: 3)
  84. Promoter Sequences - E.coli sequence & determining the promoter (reply: 4)
  85. 46 bp shift in start codon ? - tac promoter in pMAL is different from journal (reply: 1)
  86. Expression in other mammalian cells with human H1 promoter - (reply: 1)
  87. Given a sequence, how to automatically figure out where are promoters, enhancers - (reply: 1)
  88. Inserting promoters upstrem of reporter genes - (reply: 2)
  89. miRNA promoter ? - how to discover MicroRNA promoter element (reply: 1)
  90. any good websites for promoter prediction? - (reply: 7)
  91. Removing CMV promoter from pcdna3.1 - Need ideas for the same (reply: 3)
  92. inclusion of translation start site when cloning promoter insert - (reply: 3)
  93. help in cloning the promoter region of a gene - (reply: 3)
  94. Site Directed Mutagenesis within CpG rich promoter - (reply: 4)
  95. ChIP primer for promoter region of GAPDH - for taqman analysis (reply: 2)
  96. promoter methylation PCR kit from Panomics - (reply: 2)
  97. PGK promoter sequence - Somebody know how can I get it? (reply: 2)
  98. SEAP Assay for promoter activity - (reply: 2)
  99. Luciferase promoter design - (reply: 5)
  100. endogenous promoter - (reply: 1)
  101. in vitro transcription with T7 promoter - (reply: 3)
  102. How can I analysis the transcript after T7 promoter? - [HELP] (reply: 12)
  103. cloning of promoter - (reply: 2)
  104. Problem PCR amplifying gene promoter regions - (reply: 4)
  105. how the gene cloned into a vector can expressed in mammalian cell without the 5& - What is the mechanism of translation initiaition with the HCM promoter? (reply: 4)
  106. Finding promoter sequence of a gene - (reply: 2)
  107. help, methylated CMV promoter inactivated - (reply: 3)
  108. Suggestions on Research design re: Cloning the promoter region and measure induc - Molecular genetics, protocol (reply: 7)
  109. genesX promoters - (reply: 2)
  110. promoter search - (reply: 11)
  111. How to find a gene's promoter sequence? - (reply: 1)
  112. How to find the promoter sequence of this gene - (reply: 14)
  113. please suggest a program to compare promoter seuences - promoter sequences (reply: 2)
  114. T7 promoter Tm - (reply: 4)
  115. Find promoter sequence of a gene - (reply: 10)
  116. multiple copies of gene or stronger promoter..? - (reply: 2)
  117. luciferase assay promoter - include the TATA box in promoter sequence? (reply: 1)
  118. no product for promoter genomic PCR - (reply: 3)
  119. GFP or LacZ for quantification of promoter activity? - (reply: 4)
  120. cloning the promoter from genomic dna - (reply: 9)
  121. How to analyze regulator domain at promoter region - (reply: 2)
  122. promoter finding - (reply: 2)
  123. how does a promoter work - promoter (reply: 2)
  124. Free online software for comparing promoter sequences? - (reply: 1)
  125. minimal promoter - (reply: 4)
  126. How to characterize a promoter sequence - (reply: 1)
  127. How to retrieve a promoter sequences for a given gene ? - (reply: 2)
  128. How to exchange CMV for another promoter - (reply: 2)
  129. Need Help to explain a possible shared promoter - (reply: 5)
  130. promoter complex - (reply: 1)
  131. expression under fungal promoter in bacteria (?) - (reply: 4)
  132. what would happen if two SV promoter show in one vector - (reply: 1)
  133. promoter cloning - mRNBA cap site (reply: 1)
  134. inverse PCR or promoter trapping - (reply: 2)
  135. Analysis of two TF on one promoter. - (reply: 1)
  136. Use of dual promoter for siRNA - (reply: 6)
  137. Promoter recognization - to find core promoter, CAAT box, TATA box, 5' UTR,exons and 3'UTR (reply: 9)
  138. vector with H1 promoter be transfered to mouse cells? - (reply: 3)
  139. How can I change a promoter of a plasmid? - (reply: 1)
  140. promoters in Ecoli DNA - All coding regions have promoters before them? (reply: 1)
  141. promoters in Ecoli DNA - (reply: 1)
  142. how to get characterized basal promoter regions - NOT predicted ones!! (reply: 3)
  143. promoter selection in plasmid based shRNA - (reply: 2)
  144. SV40-less Luciferase promoter plasmid? - (reply: 2)
  145. How to predict a promoter region for a gene - Promoter cloning + genes into vector (reply: 6)
  146. Promoter insertion in pUAST vector - Promoter insertion in pUAST vector (reply: 1)
  147. Promoter analysis for transcription factors - (reply: 1)
  148. funtional significance of promoter SNP - (reply: 3)
  149. designing flanking primers around msp primers - (reply: 1)
  150. how many promoters be used - the number of promoters that in use (reply: 1)
  151. workflows - arabidopsis/flanking genes (reply: 1)
  152. software for promoter analysis of cis binding sites? - identifying cis-elements in promoter sequences (reply: 10)
  153. A nature article: A high-resolution map of promoters - (reply: 2)
  154. How to find promoter regulation region CpG islands - (reply: 2)
  155. Tips - how to find a promoter sequence - (reply: 4)
  156. promoter location - (reply: 6)
  157. Online sites or other programs for promoter sequence analyses? - Consensus sequences (reply: 1)
  158. PCR with T7 promoter/T7 terminator - (reply: 5)
  159. Analysis of CpG islands and promoters for methylation study - (reply: 10)
  160. Promoter Cloning - PCR amplification of a promoter and cloning (reply: 1)
  161. Finding promoter - (reply: 3)
  162. How to search and retrieve promoter sequence for methylation or ChIP study - (reply: 17)
  163. Gene promoter - (reply: 2)
  164. Promoter Prediction - (reply: 3)
  165. exception for promoter methylation-mediated silencing - (reply: 1)
  166. Bioinformatics - gene promoter regions (reply: 1)
  167. Promoter amplification.... - (reply: 7)
  168. how to find promoter region of a gene - (reply: 2)
  169. ldh-L promoter primers - Lb.plantarum (reply: 1)
  170. insert in the promoter region - (reply: 2)
  171. Promoter analysis? - (reply: 1)
  172. how to get the flanking sequences - lost, pls help (reply: 3)
  173. How to get the promoter sequence of a gene - (reply: 4)
  174. Promoter Finder - How to find out promoter of known genes (reply: 2)
  175. Green Fluroscent Protein - Identification and Characterization of Promoter usin GFP (reply: 1)
  176. How can I clone the promoter region of my gene - (reply: 3)
  177. promoter search - (reply: 3)