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flow cytometry Methods, Protocols and Troubleshootings
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Flow cytometry Related Discussions
  1. Flow cytometry on erythrocytes - Need practical advice (reply: 2)
  2. FACS overlay in WinMDI - (reply: 1)
  3. explaination of flow cytometry histogram - urgent need (reply: 4)
  4. signal amplification and facs - (reply: 1)
  5. Need guidance on FACS analysis - (reply: 3)
  6. High GFP background on FACS - (reply: 3)
  7. SOS help for GFP leakage during FACS - (reply: 2)
  8. Staning macrophages for FACS - (reply: 1)
  9. Flow cytometry by Current Protocol - free online version from invitrogen (reply: 2)
  10. T cell activation for intracellular cytokine detection by Flow Cytometry - (reply: 1)
  11. FACS data analysis - understanding cell quest program and reading data from it (reply: 1)
  12. FACS sorting into what solution for RNA isolation? - (reply: 2)
  13. GFP tagged bacterial Cell preparation for FACS analysis - (reply: 2)
  14. How to isolate Macrophages from mouse spleen and liver - for downstream functional assays by FACS (reply: 1)
  15. FACS analysis - (reply: 2)
  16. Calcium Flux by FACS using Fluo-4 - Can the cells be fixed? (reply: 1)
  17. Effect of trypsin on cells in FACS - (reply: 3)
  18. FACS 5%FBS vs 1%BSA vs FACS Buffer - (reply: 6)
  19. Which population to gate? - FACS gating question (reply: 2)
  20. to many dead cells for FACS - how to prepare "sheroid" cells gently for FACS (reply: 2)
  21. intracellular ROS with FACS - (reply: 2)
  22. Compensation in Flow cytometry! - (reply: 1)
  23. Need help in analysing FACS with WinMDI - How to edit a histogram? (reply: 1)
  24. FACS analysis with winMDI - need help in editing the histogram (reply: 1)
  25. brightest FACS fusion protein - (reply: 1)
  26. Problems with adjusting the flow cytometer - (reply: 6)
  27. help me about testing apoptosis by facs - (reply: 2)
  28. Looking for primary antibody to BiP (grp78) for flow cytometry - (reply: 2)
  29. quantifying TUNEL: flow cytometry vs direct counting on slides? - (reply: 2)
  30. Flow cytometry Stats - Median, Arithmetic Mean, Geometric Mean (reply: 2)
  31. Macrophage flow cytometry - (reply: 1)
  32. FACS instrument prices URGENT - need your advice on FACS (reply: 1)
  33. high self fluorescence when doing FACS - (reply: 3)
  34. interpretation of FACS data - (reply: 2)
  35. storing unstained cells overnight for FACS next day - (reply: 3)
  36. FACS sorting and culturing cells afterwards - (reply: 1)
  37. Help with my FACS histograms, please - (reply: 5)
  38. Frustrated with FACS (ONLY DEAD CELLS) - (reply: 8)
  39. how do i do flow cytometry for adherent cells? - (reply: 5)
  40. stainig of cells for FACS - (reply: 2)
  41. any tips for detecting phospho-ptotein in fixed adherent cells by flow cytometry - (reply: 2)
  42. FLAG antibodies for mouse samples - Immunofluorescence/Flow cytometry (reply: 1)
  43. FACS sorting affects cell function? - FACS sorting affects cell function (reply: 2)
  44. FACS PI staining for DNA content analysis - instrumental settings (reply: 1)
  45. difference between propylene and polystyrene tubes, for Cytometry? - propylene orpolystyrene tubes for Cytometry? (reply: 2)
  46. human blood cytometry - (reply: 4)
  47. FACS GFP leakage - (reply: 4)
  48. How get GFP signal without mRNA target from pIRES-EGFP - positive by FACS but no DNA amplifation from RT-PCR (reply: 8)
  49. beads for compensation controls for FACS - (reply: 1)
  50. FACS markers for adipocytes and osteoblasts - Would like to separate adipocytes/fibroblasts from bone marrow with FA (reply: 1)
  51. cd3- and cd8+ cells - flow cytometry cell population question (reply: 4)
  52. How small cells can Flow cytometry detect? - Flow cytometry to detect a small cell (reply: 2)
  53. Duration of the signal for flow cytometry - (reply: 2)
  54. Investigating apoptosis by flow cytometry - (reply: 6)
  55. Flow Cytometry 2 Parameter Statistics, please help - Can you find standard deviation of % Positive of Total cells based on (reply: 1)
  56. FACS Aria Sorter - Concentrating the sorterd polyplex sample (reply: 1)
  57. Permeabilisation for PI staining in FACS? - (reply: 2)
  58. general flow cytometry protocol - (reply: 3)
  59. FACS analysis - (reply: 1)
  60. FACS analysis - (reply: 1)
  61. FITC and PE Overlapping emissions in Flow Cytometry - (reply: 2)
  62. About to run FACS: But I forgot my single stain controls! - (reply: 1)
  63. Primary cultures of breast carcinoma for FACS - (reply: 1)
  64. Apoptosis assay - other than FACS (reply: 2)
  65. FACS Protocol Help! - (reply: 9)
  66. Flow Cytometry Intracellular and dead cell staining - (reply: 2)
  67. How to disassociate cells for FACS? - (reply: 7)
  68. Sub-G1 FACS analysis - basic question (reply: 6)
  69. how to analyze facs results results - (reply: 1)
  70. Help with a project on flow cytometer - (reply: 2)
  71. isolating RNA from FACS - (reply: 2)
  72. labeling of cytokine releasing cells - flow cytometry (reply: 7)
  73. Longer time and higher temperature for flow cytometry. - what will happen (reply: 2)
  74. need help in facs data analysis of surface expression - (reply: 3)
  75. Free FACS analysis software for PC - (reply: 20)
  76. CCR5 cytometry on dendritic cells, probles with isotype control - (reply: 2)
  77. FACS of stimulated human PBMC - (reply: 2)
  78. Removing adherent cells for Flow cytometry without trypsin - how to do it (reply: 3)
  79. FACS and isotypes - (reply: 3)
  80. Storage of cell samples before flow cytometry - (reply: 3)
  81. Cell cycle analysis (FACS) analysis and GFP detection - (reply: 10)
  82. Basic question on FACS - (reply: 4)
  83. flow cytometry problem - (reply: 3)
  84. Which is best room for FACS Analysis discussion? - (reply: 4)
  85. FACS analysis of receptor surface expression using fluorescent antibodies - (reply: 3)
  86. Cell detachment for flow cytometry expts - (reply: 5)
  87. Apoptag Red for Flow Cytometry - (reply: 1)
  88. Flow Cytometry Questions - debris removal and G2/M visualization (reply: 1)
  89. Fluorophore exc505-emiss515nm-possible to use? - with 488 argon laser? (FACS, microscope?) (reply: 1)
  90. isotype-control in FACS shifts heavily - (reply: 7)
  91. DNA stain for viable FACS cell sorting? - (reply: 7)
  92. Flow cytometry - permeabilization protocol? - (reply: 1)
  93. FACS control high background - more stained cells in control than in sample (reply: 4)
  94. can I re-freeze the 4% paraformaldehyde for longer storage? - leave cells in 4% paraformaldehyde 4 FACS? (reply: 3)
  95. Transfection, GFP, FACS and UV Microscope - great inconsistency b/t FACS and UV microscope (reply: 3)
  96. mouse Fibroblast specific surface markers??? - how to gate out mouse Fibroblast cells in FACS (reply: 1)
  97. FACS: DNA content and GFP in one go!!! - PI masks GFP??? (reply: 4)
  98. Cell cycle (phase) analysis by FACS analysis - PI staining to gate different phases of cell cycle (reply: 1)
  99. Help with Flow cytometry - (reply: 2)
  100. flow cytometry and SP analysis - (reply: 2)
  101. Flow Cytometry Analysis: Linux/Unix - (reply: 7)
  102. BrdU assay by FACS - (reply: 1)
  103. Need advice on FACS for cell cycle analysis - (reply: 3)
  104. Flow Cytometry: How to gate Apoptosis? - FSC / SSC Dotplot (reply: 1)
  105. Alexa to replace PE - For flow cytometry (reply: 2)
  106. FACS analysers - Purchase of a FACS analyser and cell sorter (reply: 3)
  107. FACS analysis in adherent cell lines - FACS analysis in adherent cell lines (reply: 3)
  108. FACS analysis with CD49d From Pharmingen - FACS with CD49d (reply: 1)
  109. FACS analysis for tissue samples? - (reply: 2)
  110. Ca2+ or no Ca2+ in FACS medium? - discussion (reply: 1)
  111. lymphocytes in FACS - (reply: 1)
  112. Plant cell preparation - Flow cytometry (reply: 1)
  113. Flow Cytometry - (reply: 1)
  114. FACS microparticles - (reply: 1)
  115. Flow cytometry analysis - aggregates? - Analysis of aggregates (reply: 1)
  116. Flow Cytometry Analysis on PC - Anyone know of software (reply: 8)
  117. FACS - Is there an alternative for FITC to label E. coli for FACS (reply: 1)
  118. Detection of IntracellularStaining using Flow cytometry - (reply: 2)
  119. Flow cytometry - (reply: 2)