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JoVE: Counting and Determining the Viability of Cultured Cells (Video Protocol)

Journal of Visualized Experiments

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Counting and Determining the Viability of Cultured Cells

Richard Ricardo, Katy Phelan
Molecular Pathology Laboratory Network, Inc
Determining the number of cells in culture is important in standardization of culture conditions and in performing accurate quantitation experiments. A hemacytometer is a thick glass slide with a central area designed as a counting chamber. Cell suspension is applied to a defined area and counted so cell density can be calculated.

The complete text protocol for this experimental approach is available in Current Protocols in Cell Biology.

Ricardo R, Phelan K (2008). Counting and Determining the Viability of Cultured Cells JoVE. 16. http://www.jove.com/index/details.stp?id=752, doi: 10.3791/752