Department of Biological Sciences,

1000 Hilltop Circle

Baltimore, MD 21250

Beginning Molecular Biology Laboratory Manual

CHAPTER 1: General Laboratory Methods

CHAPTER 2: Instructions for Notebook Keeping

CHAPTER 3: Vector NTI User's Guide

CHAPTER 4: Molecular Biology Methods

• Preparation of genomic DNA from bacteria
• PCR amplification of DNA
• Restriction enzyme digestion of DNA
• Phenol/chloroform extraction of DNA
• Ethanol precipitation of DNA
• Agarose gel electrophoresis
• Transformation of E. coli by electroporation
• Preparative DNA Fragment Isolation from an Agarose Gel
• Ligations of plasmid DNA to insert DNA
• Transfection of mammalian cells using Lipofectamine
• Southern blotting
• Western Blot analysis of His-tagged proteinsl
• Cycle Sequencing Protocols For ABI 3100
• Colony PCR
• One Step Gene Assembly (Gene Synthesis)

• Types of cells grown in culture
• Work area and equipment
• Preservation and storage
• Maintenance
• Safety considerations
• Tissue culture procedures
• Determining cell counts

Common Stock Solutions

Visit the Course Wiki page for more protocols

This Web page is maintained by Julie B. Wolf, UMBC;
Last updated on 1/18/2006