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Immunization Protocol for Monoclonal Antibody Production
Antigens:
Proteins: 10-100 µg
Cells: 0.5 - 5.0 x107
Adjuvant:
Complete Freunds Adjuvant
None
Schedule:
Day | Manipulation P> | Adjuvant | Site |
0 | Primary Immunization | CFA | s.c. |
14 | Boost #1 | IFA | s.c. |
24 | Boost #2 | IFA | s.c. |
35 | Boost #3 | IFA | i.v. |
42 | Bleed and test for antibody | ||
45 | Boost #4 | IFA | i.v. |
66+ | 3-4 week rest followed by i.v. or i.p. boost | Saline/PBS | i.v. |
69 | fusion 3d after i.v. boost or 4d after i.p. boost | -- | i.v. |