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Formaldehyde Treatment of Tissue Culture Hoods

Formaldehyde Treatment of Tissue Culture Hoods

You will need:-

12g Potassium Permanganate
6g Crushed paraformaldehyde

1) Set the hood so that it can vent outside.

2) Mix the two chemicals above together (in a jam/coffee jar, so it can be thrown away) in the hood, wear gloves and a face mask.

N.B.: Potassium permanganate is a strong oxidising agent while paraformaldehyde and formaldehyde vapour are extremely toxic.

3) Add 50ml water and mix.

4) Quickly place the front door of the hood in position, and tape over the joints.

5) Leave overnight and then switch on hood for at least 1 hour prior to use.

Keywords: Formaldehyde, tissue culture
Submitted at 11:36 on 16/2/96 by:

Jill Fergusson,
Department of Biochemistry,
University of Nottingham,
The Medical School ,
Q.M.C. ,
Clifton Boulevard,
NG7 2UH,
U.K..

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