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Serum Separation from Whole Blood

Serum Separation from Whole Blood

1) Collect sample (preferably in glass tubes) and leave for 1 hour at 37°C to allow it to clot.

2) Leave sample at 4°C overnight to allow the clot to contract.

3) Using a glass pasteur carefully loosen the clot from the sides of the tube. It is important not to lyse the red cells as they can not then be separated from the serum.

4) Centrifuge the serum at 4000 rpm for 20 minutes @ 4°C.

5) Remove the serum from the clot by gently pipetting off into a clean tube using a glass pasteur.

6) Label with the animal number, antigen, total bleed and date.

7) Store at -20°C.

Keywords: serum, blood, separation
Submitted at 13:54 on 22/2/96 by:

Jill Fergusson,
Department of Biochemistry,
University of Nottingham,
The Medical School ,
Q.M.C. ,
Clifton Boulevard,
NG7 2UH,
U.K..

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