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Native Acrylamide Gels

Native Acrylamide Gels (35 ml)

  3.5% 5% 6% 8%
30/0.8% acrylamide 4.1 ml 5.8 ml 7 ml 9.3 ml
10X TBE 3.5 ml 3.5 ml 3.5 ml 3.5 ml
H20 27.4 ml 25.7 ml 24.5 ml 22.2 ml

Degas 1-2 min

Add 0.25 ml 10% ammonium persulfate

Add 30 microliters TEMED

Pour Gel and wait 20-30 minutes before removing gel comb

Run at 180 volts in 1X TBE Buffer

 

Steve Hahn

Last modified Fri, Oct 16, 1998

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