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Footprinting

Footprinting

Footprinting is a method for determining the exact DNA sequence to which a particular DNA-binding protein binds. Examples: How, for example, does one determine the DNA sequence to which the lac repressor binds?

The procedure:

Link to discussion of DNA sequencing.
Link to a view of the actual sequence of the lac operator
Link to a discussion of how transcription factors can be isolated from the cell.
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7 August 2003