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Aseptic Technique for Tissue Culture

Aseptic Technique for Tissue Culture

By John Jones last modified 16/06/2003
The use of aseptic technique is essential for avoiding the production of infection whilst undertaking tissue culture activities.

Many activities take place within a laminar flow hood, either Class I (use with animal tissues), or Class II (use with human tissues).

The purpose of these hoods is to minimise the risk of infection entering from the outside environment, as well as protect the user against potential pathogens being transmitted from the culture into their environment.

A clean hood

Before using a hood it is important that it is cleaned. This is typically by means of a chemical such as Industrial Methylated Spirits (IMS).

Cleaning the hood by means of swabbing with IMS or other suitable chemicals is very important. All items entering the hood from that moment on should also be treated with IMS to ensure sterility.

An uncluttered workspace

Reaching over items has a chance of knocking things over, or transfering infection through the workplace. For this reason it is good practice to plan the layout of the workspace before use. Should anything be spilled, mopping with IMS may minimise potential problems.

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