This is a cached page for the URL (http://www.synthegen.com/technical/protocols/anneal.lasso). To see the most recent version of this page, please click here.
Protocol Online is not affiliated with the authors of this page nor responsible for its content.
About Cache
Annealing Oligonucleotides
 
Username
 
Password
 
 
Home | Products & Services | How to Order | Request Quote | Technical Support | Contact Information | Register
Search: Advanced Search
Quick Help:
 

Protocol for Annealing Oligonucleotides

1. Prepare a solution of 10X annealing buffer.

10 mM MgCl2, 200 mM Tris-HCl, pH 8.0

2. Prepare an aliquot of the oligonucleotide probe in an eppendorf tube.

3. Add a molar excess of oligonucleotide target to the tube.

4. Calculate the volume of 10X annealing buffer to add.

Reaction Volume = Volume of Probe + Volume of Target

Volume of 10X Annealing Buffer = 9 x Reaction Volume

5. Add the 10 X Annealing buffer and vortex to mix.

6. Place the tube in a 95oC Heat Block for 3 minutes.

7. Remove the tube and let it cool to room temperature. (~10 minutes)

8. Vortex the mixture

 

Home | Products & Services | How to Order | Request Quote
Technical Support | Contact Information | Search

Privacy Policy | Website Comments or Suggestions?
© 1996-2002 by SYNTHEGEN, LLC. All rights Reserved.